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Our Abpromise guarantee covers the use of ab109824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Neutralising||Use a concentration of 0.1 - 0.2 µg/ml. to yield one-half maximal inhibition inhibition [ND50] of the biological activity of Human TWEAK (200 ng/ml)|
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 27 kDa. The detection limit for recombinant Human TWEAK is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|Indirect ELISA||Use a concentration of 0.5 - 2 µg/ml. ab109824 allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TWEAK (using 100 µl/well antibody solution).|
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to TWEAK (Biotin) (ab83437). ab109824 in conjunction with ab83437 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TWEAK (using 100 µl/well antibody solution).|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"