The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Negatively regulates SHH signal transduction (By similarity). Probably functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to phosphorylated inositide lipids.
組織特異性Expressed at high levels in testis, ovaries, thyroid, and spinal chord.
配列類似性Belongs to the TUB family.
細胞内局在Nucleus. Cell membrane. Cell projection > cilium. Cytoplasm. Secreted. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway (By similarity). Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha.