製品の概要

  • 製品名Anti-Tubulin antibody [DM1A +DM1B]
    Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [DM1A +DM1B] to Tubulin
  • アプリケーション適用あり: Electron Microscopy, ICC/IF, IP, IHC-P, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Drosophila melanogaster
    交差が予測される動物種: Chicken, Guinea pig, Cow, Pig, Gerbil
  • 免疫原

    Tissue/ cell preparation, Native chick brain microtubules.

  • エピトープaa 426-450
  • ポジティブ・コントロール
    • In Western Blot, this antibody gave a positive signal in the following whole cell lysates: HeLa; NIH3T3; PC12. Ls174T, MAD109 cells. Skin or lung.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab44928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Electron Microscopy Use at an assay dependent concentration.
ICC/IF 1/2000.
IP 1/400.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 50 kDa).
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

Anti-Tubulin antibody [DM1A +DM1B] 画像

  • Ab44928 staining human normal placenta. Staining is localized to the cytoplamsm
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab44928 staining Tubulin in Drosophila S2 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 5% BSA for 15 minutes at Room temperature. Samples were incubated with primary antibody (1/2000 in 5% BSA + PBST) for 2 hours. Ab6785 (1/1000) was used as secondary antibody.

    See Abreview

  • All lanes : Anti-Tubulin antibody [DM1A +DM1B] (ab44928) at 1/5000 dilution

    Lane 1 : Whole cell lysate prepared from human colon cells
    Lane 2 : Whole cell lysate prepared from human colon cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP sheep anti-mouse polyclonal at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Image courtesy of Qifeng Lin by Abreview.

    See Abreview

  • All lanes : Anti-Tubulin antibody [DM1A +DM1B] (ab44928) at 5 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 50 kDa
    Observed band size : 53 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
  • Overlay histogram showing HeLa cells stained with ab44928 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab449287, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-Tubulin antibody [DM1A +DM1B] (ab44928) 使用論文

This product has been referenced in:
  • Newton FG  et al. Coordinate post-transcriptional repression of Dpp-dependent transcription factors attenuates signal range during development. Development 142:3362-73 (2015). WB ; Drosophila melanogaster . Read more (PubMed: 26293305) »
  • Uddin M  et al. Integrated genomics identifies convergence of ankylosing spondylitis with global immune mediated disease pathways. Sci Rep 5:10314 (2015). WB . Read more (PubMed: 25980808) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Zebrafish Cell lysate - whole cell (brain tissue)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 5 µg
Specification brain tissue
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

投稿 Jun 19 2015

Application Western blot
Sample Nothobranchius furzeri Cell lysate - whole cell (brain)
Gel Running Conditions Reduced Denaturing (12%)
Loading amount 5 µg
Specification brain
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Tyrone Genade

Verified customer

投稿 Jun 18 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Tissue sections (Left atrial appendage)
Specification Left atrial appendage
Permeabilization No
Fixative Paraformaldehyde
Username

Dr. Beth Bunnell

Verified customer

投稿 Sep 08 2014

Western blot

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (4-20%)
Sample Human Cell lysate - other (A375 melanoma cells)
Specification A375 melanoma cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Jun 10 2013

Thank you for these details.

I appreciate the time you have spent on these experiments and would be pleased to arrange a credit note or a replacement with a different lot number of ab44928 or with an alternative anti-tubulin form our catalogue...

Read More

Thank you for contacting us.

I am not sure I understand the problem you are experiencing with the anti-tubulin ab44928.
Are you observing an extra band in Western Blot after immunoprecipitation?
What type of sample are you working on? ...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products.
We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I a...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (retina or eyecup without retina, also rat cells in)
Loading amount 20 µg
Specification retina or eyecup without retina, also rat cells in
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Aug 13 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Colon)
Loading amount 20 µg
Specification Colon
Gel Running Conditions Reduced Denaturing (12.5)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Qifeng LIN

Verified customer

投稿 Jul 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Fruit fly (Drosophila melanogaster) Cell (Drosophila S2 cells)
Specification Drosophila S2 cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: R.T°C
Username

Ms. Maureen Sinclair

Verified customer

投稿 Feb 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"