製品の概要

  • 製品名Anti-Tubulin antibody
    Tubulin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Tubulin
  • アプリケーション適用あり: WB, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Rat, Rabbit, Human
  • 免疫原

    Recombinant full length protein, corresponding to amino acids 1-452 of Human Tubulin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab56676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.



ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Tubulin beta 2b antibody
    • Alpha tubulin antibody
    • Alpha-tubulin ubiquitous antibody
    • beta Ib tubulin antibody
    • CDCBM5 antibody
    • CDCBM6 antibody
    • fd02b12 antibody
    • K ALPHA 1 antibody
    • M40 antibody
    • OK/SW-cl.56 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • TUBB1 antibody
    • TUBB2 antibody
    • TUBB5 antibody
    • tubulin alpha 1b antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin beta 1b antibody
    • Tubulin beta 2A antibody
    • tubulin beta 2A class IIa antibody
    • Tubulin beta antibody
    • tubulin beta chain antibody
    • tubulin beta class I antibody
    • tubulin beta-1 chain antibody
    • tubulin beta-2A chain antibody
    • tubulin beta-5 chain antibody
    • Tubulin K-alpha-1 antibody
    • tubulin, alpha, ubiquitous antibody
    • tubulin, beta 2A class IIa antibody
    • tubulin, beta polypeptide 2 antibody
    • tubulin, beta polypeptide antibody
    • tubulin, beta, class IIA antibody
    • wu:fd02b12 antibody
    • zgc:55461 antibody
    see all

Anti-Tubulin antibody 画像

  • Tubulin antibody (ab56676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human prostate.
  • Tubulin antibody (ab56676) at 1ug/lane + A-431 cell lysate at 25ug/lane.
  • ab56676 at 1/500 dilution staining Tubulin in Human Osteosarcoma cells by immunocytochemistry/ Immunofluorescence. The cells were PFA fixed, permeabilized in PBS (0.5% Triton) and blocked in 1% BSA for 1 hour at 25°C. An Alexa Fluor® 488 conjugated Goat polyclonal to mouse IgG at 1/250 dilution, was used as secondary antibody. The primary antibody shows green staining in image.

    See Abreview

  • All lanes : Anti-Tubulin antibody (ab56676) at 1 µg/ml (in TPBS for 18 hours at 4°C)

    Lane 1 : Whole tissue lysate of rabbit heart at 20 µg
    Lane 2 : Whole tissue lysate of rabbit heart at 40 µg
    Lane 3 : Whole tissue lysate of rabbit heart at 60 µg
    Lane 4 : Whole tissue lysate of rabbit heart at 80 µg
    Lane 5 : Whole tissue lysate of rabbit heart at 100 µg
    Lane 6 : Whole tissue lysate of rabbit heart at 120 µg

    Secondary
    An HRP-conjugated Donkey anti-mouse IgG polyclonal at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 47 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second

    This image is courtesy of an Abreview submitted by Xun Ai

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab56676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56676, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-Tubulin antibody (ab56676) 使用論文

This product has been referenced in:
  • Neirinckx V  et al. Adult bone marrow mesenchymal and neural crest stem cells are chemoattractive and accelerate motor recovery in a mouse model of spinal cord injury. Stem Cell Res Ther 6:211 (2015). WB ; Mouse . Read more (PubMed: 26530515) »
  • Mauger O  et al. Alternative splicing regulates the expression of G9A and SUV39H2 methyltransferases, and dramatically changes SUV39H2 functions. Nucleic Acids Res 43:1869-82 (2015). WB . Read more (PubMed: 25605796) »

See all 9 Publications for this product

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (PC12)
Specification PC12
Fixative Formaldehyde
Permeabilization Yes - 0.2% Triton X100
Blocking step Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rabbit Tissue lysate - whole (heart)
Loading amount 20 µg
Specification heart
Gel Running Conditions Reduced Non-Denaturing (Native) (10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Dr. Xun Ai

Verified customer

投稿 Sep 14 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (osteosarcoma cell line)
Loading amount 15 µg
Specification osteosarcoma cell line
Gel Running Conditions Reduced Denaturing (page 10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. CESAR CAMACHO

Verified customer

投稿 May 18 2010

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (osteosarcoma cell line)
Specification osteosarcoma cell line
Fixative Paraformaldehyde
Permeabilization Yes - pbs-triton .5%
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 May 18 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"