The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/3000 is determined for one of the three peptides.
WB: 1/100. Predicted molecular weight: 59 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.