遺伝子ノックアウト細胞株 検証済
リコンビナント
RabMAb

Anti-Tuberin 抗体 [Y320] (ab32554)

製品の概要

  • 製品名
    Anti-Tuberin antibody [Y320]
    Tuberin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y320] to Tuberin
  • 特異性
    ab32554 recognises Tuberous sclerosis complexe-2 (TCS2 also known as Tuberin. It is predicted to detect isoform 2-4 based on sequence homology.
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cytmore details
    適用なし: ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C-term of human TSC2.

  • ポジティブ・コントロール
    • WB: Jurkat cell lysate IHC: prostate carcinoma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 220 kDa (predicted molecular weight: 200 kDa).
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 追加情報
    Is unsuitable for ICC/IF or IP.
  • ターゲット情報

    • 機能
      In complex with TSC1, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1. Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling. Stimulates weakly the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 in vitro. Mutations in TSC2 lead to constitutive activation of RAP1A in tumors.
    • 組織特異性
      Liver, brain, heart, lymphocytes, fibroblasts, biliary epithelium, pancreas, skeletal muscle, kidney, lung and placenta.
    • 関連疾患
      Defects in TSC2 are the cause of tuberous sclerosis type 2 (TSC2) [MIM:613254]. TSC2 is an autosomal dominant multi-system disorder that affects especially the brain, kidneys, heart, and skin. It is characterized by hamartomas (benign overgrowths predominantly of a cell or tissue type that occurs normally in the organ) and hamartias (developmental abnormalities of tissue combination). Clinical symptoms can range from benign hypopigmented macules of the skin to profound mental retardation with intractable seizures to premature death from a variety of disease-associated causes.
      Defects in TSC2 are a cause of lymphangioleiomyomatosis (LAM) [MIM:606690]. LAM is a progressive and often fatal lung disease characterized by a diffuse proliferation of abnormal smooth muscle cells in the lungs. It affects almost exclusively young women and can occur as an isolated disorder or in association with tuberous sclerosis complex.
    • 配列類似性
      Contains 1 Rap-GAP domain.
    • 翻訳後修飾
      Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1.
      Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation.
    • 細胞内局在
      Cytoplasm. Membrane. At steady state found in association with membranes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • FLJ43106 antibody
      • LAM antibody
      • OTTHUMP00000158940 antibody
      • OTTHUMP00000198394 antibody
      • OTTHUMP00000198395 antibody
      • PPP1R160 antibody
      • Protein phosphatase 1, regulatory subunit 160 antibody
      • TSC complex subunit 2 antibody
      • tsc2 antibody
      • TSC2_HUMAN antibody
      • TSC4 antibody
      • TSC4 gene, formerly antibody
      • TSC4, formerly antibody
      • Tuberin antibody
      • Tuberous sclerosis 2 antibody
      • Tuberous sclerosis 2 protein antibody
      • Tuberous sclerosis 2 protein homolog antibody
      see all

    Anti-Tuberin antibody [Y320] 画像



    • Predicted band size : 200 kDa

      Lane 1: Wild type HAP1 whole cell lysate (20 µg)              
      Lane 2: Tuberin knockout  HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32554 observed at 180 kDa. Red - loading control, ab18058, observed at 130 kDa.

      ab32554 was shown to specifically react with tuberin when tuberin knockout samples were used. Wild-type and tuberin knockout samples were subjected to SDS-PAGE.  Ab32554 and ab18058 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Ab32554 at a 1:50 dilution staining Tuberin in human prostate carcinoma.

    • Anti-Tuberin antibody [Y320] (ab32554) at 1/1000 dilution + Jurkat cell lysate

      Predicted band size : 200 kDa
      Observed band size : 220 kDa (why is the actual band size different from the predicted?)
    • Overlay histogram showing HepG2 cells stained with ab32554 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32554, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    Anti-Tuberin antibody [Y320] (ab32554) 使用論文

    This product has been referenced in:
    • Pal R  et al. Inhibition of ERK1/2 Restores GSK3ß Activity and Protein Synthesis Levels in a Model of Tuberous Sclerosis. Sci Rep 7:4174 (2017). Read more (PubMed: 28646232) »
    • D'Armiento J  et al. Mesenchymal Tumorigenesis Driven by TSC2 Haploinsufficiency Requires HMGA2 and Is Independent of mTOR Pathway Activation. Cancer Res 76:844-54 (2016). Read more (PubMed: 26837766) »

    See all 10 Publications for this product

    Product Wall

    All three antibodies will detect the proteins with or without phosphorylation. The one exception may be the TSC2 antibody, which is raised against a C terminus peptide that contains a phosphorylation site at serine 1798. It is possible that if this ami...

    Read More

    Muchas gracias por contactarnos.

    Anticuerpos contra HAMARTINA, o TSC1 testados en IHC-P, tenemos cuatro, dos de conejo policlonal, uno monoclonal también de conejo, y uno policlonal de cabra. En función de las especies en las qu...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa)
    Loading amount
    20 µg
    Specification
    HeLa
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Dr. jp lu

    Verified customer

    投稿 Nov 21 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    登録