製品の概要

  • 製品名Anti-Tubby antibody
    Tubby 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tubby
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Tubby.

    (Peptide available as ab47529.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Human Spinal Cord, Mouse Brain, Mouse Testis, Mouse Spinal Cord, Rat Brain and Rat Dorsal Root Ganglion.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab46943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

  • 関連性Tubby is a bipartite transcription factor. It may play a role in obesity and sensorineural degradation. The crystal structure has been determined for a similar protein in mouse, which functions as a membrane bound transcription regulator that translocates to the nucleus in response to phosphoinositide hydrolysis.
  • 細胞内局在Cytoplasm. Nucleus. Secreted. Cell membrane. Binds phospholipid and is anchored to the plasma membrane through binding phosphatidylinositol 4,5-bisphosphate. Is released upon activation of phospholipase C. Translocates from the plasma membrane to the nucleus upon activation of guanine nucleotide-binding protein G(q) subunit alpha. Does not have a cleavable signal peptide and is secreted by a non-conventional pathway.
  • 参照データベース
  • 別名
    • Mouse tubby homologue antibody
    • rd5 antibody
    • Retinal degeneration 5 antibody
    • TUB antibody
    • Tubby (mouse) homolog antibody
    • tubby antibody
    • Tubby homolog (mouse) antibody
    • Tubby homologue antibody
    • Tubby protein homolog antibody
    see all

Anti-Tubby antibody 画像

  • All lanes : Anti-Tubby antibody (ab46943) at 1 µg/ml

    Lane 1 : Human spinal cord tissue lysate - total protein (ab29188)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate - normal tissue
    Lane 4 : Spinal Cord (Mouse) Tissue Lysate
    Lane 5 : Brain (Rat) Tissue Lysate - normal tissue
    Lane 6 : Rat Dorsal Root Ganglion

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • IHC image of Tubby staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab46943, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Tubby antibody (ab46943) 使用論文

ab46943 has not yet been referenced specifically in any publications.

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They confirmed the mouse strain used is CD-1 and the animals used are surplus animals over 8 weeks old.

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The lysates were prepared using RIPA buffer and phosphatase inhibitors. The gel was run under reducing conditions, and the blocking was pe...

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