Anti-TUBB2A + TUBB2B 抗体 [EPR11843] (ab170931)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11843] to TUBB2A + TUBB2B
- Suitable for: Flow Cyt (Intra), WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TUBB2A + TUBB2B antibody [EPR11843]
TUBB2A+TUBB2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11843] to TUBB2A + TUBB2B -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, IPmore details
適用なし: ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Recombinant fragment within Human TUBB2A. The exact sequence is proprietary.
Database link: Q13885 -
ポジティブ・コントロール
- 293T, HepG2, Jurkat and HeLa whole cell lysate (ab150035); Human brain tissue; Jurkat cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR11843 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab170931の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/10000 - 1/50000. Predicted molecular weight: 50 kDa.
|
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
IP |
1/10 - 1/100.
|
特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/10000 - 1/50000. Predicted molecular weight: 50 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
1/10 - 1/100. |
ターゲット情報
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細胞内局在
TUBB2A: Cytoplasm > cytoskeleton. TUBB2B: Cytoplasm, cytoskeleton. -
参照データベース
- Entrez Gene: 347733 Human
- Entrez Gene: 7280 Human
- Entrez Gene: 22151 Mouse
- Entrez Gene: 73710 Mouse
- Entrez Gene: 291081 Rat
- Entrez Gene: 498736 Rat
- Omim: 612850 Human
- SwissProt: Q13885 Human
see all
画像
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All lanes : Anti-TUBB2A + TUBB2B antibody [EPR11843] (ab170931) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 50 kDa -
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling TUBB2A with ab170931 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from 293T cell lysate (Lane 1) or 1X PBS (negative control) (Lane 2) using ab170931 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Lane 1 Primary: ab170931, 1/10000 dilution
Sample: Immunoprecipitation pellet from 293T cell lysate
Secondary: HRP-conjugated anti-rabbit IgGLane 2 Primary: ab170931, 1/10000 dilution
Sample: 1X PBS (negative control)
Secondary: HRP-conjugated anti-rabbit IgG -
Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling TUBB2A with ab170931 at 1/10 dilution (red) compared toa rabbit IgG negative control (green).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab170931 は 4 報の論文で使用されています。
- Jin Y et al. Circular RNA circ_C16orf62 Suppresses Cell Growth in Gastric Cancer by miR-421/Tubulin beta-2A Chain (TUBB2A) Axis. Med Sci Monit 26:e924343 (2020). PubMed: 33006960
- Klepinin A et al. The complexity of mitochondrial outer membrane permeability and VDAC regulation by associated proteins. J Bioenerg Biomembr N/A:N/A (2018). PubMed: 29998379
- Zhang X et al. Analysis of Liver Tumor-Prone Mouse Models of the Hippo Kinase Scaffold Proteins RASSF1A and SAV1. Cancer Res 76:2824-35 (2016). PubMed: 26980762
- Chekulayev V et al. Metabolic remodeling in human colorectal cancer and surrounding tissues: alterations in regulation of mitochondrial respiration and metabolic fluxes. Biochem Biophys Rep 4:111-125 (2015). PubMed: 29124194