製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TUBA8
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 32-81 (ADGTFGTQAS KINDDDSFTT FSETGNGKHV RAVMVDLEPT VDEVRAG) of Mouse TUBA8 (NP_059075).

  • ポジティブ・コントロール
    • Mouse spleen lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Constituents: 98% PBS, 2% Sucrose
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab113921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 組織特異性
    Preferentially expressed in heart, skeletal muscle and testis. Expressed at low levels in the developing brain.
  • 関連疾患
    Defects in TUBA8 are the cause of polymicrogyria with optic nerve hypoplasia (PMGONH) [MIM:613180]. It is a disease characterized by extensive polymicrogyria, optic nerve hypoplasia, severe developmental delay, hypotonia, seizures, a dysplastic or absent corpus callosum and colpocephaly. Polymicrogyria is a malformation of the cortex in which the brain surface is irregular and characterized by an excessive number of small gyri with abnormal lamination.
  • 配列類似性
    Belongs to the tubulin family.
  • 翻訳後修飾
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
  • 細胞内局在
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha tubulin 8 antibody
    • Alpha-tubulin 8 antibody
    • TBA8_HUMAN antibody
    • Tuba8 antibody
    • TUBAL2 antibody
    • Tubulin alpha 8 chain antibody
    • Tubulin alpha chain like 2 antibody
    • Tubulin alpha chain-like 2 antibody
    • Tubulin alpha-8 chain antibody
    see all

画像

  • Anti-TUBA8 antibody (ab113921) at 1 µg/ml + Mouse spleen lysate at 10 µg

    Predicted band size : 50 kDa

プロトコール

参考文献

ab113921 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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