製品の概要

  • 製品名Anti-TUBA1B antibody
    TUBA1B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TUBA1B
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 118-375 of Human TUBA1B (NP_006073)

  • ポジティブ・コントロール
    • HeLa, A431, 293T, H1299 and Raji cells.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab97622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha tubulin ubiquitous antibody
    • Alpha-tubulin ubiquitous antibody
    • K alpha 1 antibody
    • TBA1B_HUMAN antibody
    • TUBA1B antibody
    • Tubulin alpha 1B antibody
    • Tubulin alpha 1B chain antibody
    • Tubulin alpha ubiquitous antibody
    • Tubulin alpha ubiquitous chain antibody
    • Tubulin alpha-1B chain antibody
    • Tubulin alpha-ubiquitous chain antibody
    • Tubulin K alpha 1 antibody
    • Tubulin K-alpha-1 antibody
    see all

Anti-TUBA1B antibody 画像

  • Anti-TUBA1B antibody (ab97622) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 50 kDa
  • Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab97622 at 1/200 dilution. Bottom picture shows stain merged with DNA probe.

Anti-TUBA1B antibody (ab97622) 使用論文

ab97622 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"