製品の概要

  • 製品名Anti-TUBA1A antibody
    TUBA1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TUBA1A
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 37-286 of Human TUBA1A (NP_006000).

  • ポジティブ・コントロール
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate. A431 cells

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 組織特異性Expressed at a high level in fetal brain.
  • 関連疾患Lissencephaly 3
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在Cytoplasm, cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha tubulin 3 antibody
    • Alpha-tubulin 3 antibody
    • B alpha 1 antibody
    • FLJ25113 antibody
    • LIS3 antibody
    • TBA1A_HUMAN antibody
    • TUBA1A antibody
    • TUBA3 antibody
    • Tubulin alpha 1a antibody
    • Tubulin alpha 1A chain antibody
    • Tubulin alpha 3 antibody
    • Tubulin alpha 3 chain antibody
    • Tubulin alpha brain specific antibody
    • Tubulin alpha-1A chain antibody
    • Tubulin alpha-3 chain antibody
    • Tubulin B alpha 1 antibody
    • Tubulin B-alpha-1 antibody
    see all

Anti-TUBA1A antibody 画像

  • All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
  • ab95966 at 1/200 dilution staining TUBA1A in paraformaldehyde-fixed A431 cells by immunofluorescence. ab95966 has been merged with a DNA probe in the lower image.

Anti-TUBA1A antibody (ab95966) 使用論文

ab95966 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"