Anti-TUBA1A 抗体 (ab95966)
Key features and details
- Rabbit polyclonal to TUBA1A
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Drosophila melanogaster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-TUBA1A antibody -
製品の詳細
Rabbit polyclonal to TUBA1A -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, Drosophila melanogaster -
免疫原
Recombinant fragment, corresponding to a region within amino acids 37-286 of Human TUBA1A (NP_006000).
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ポジティブ・コントロール
- WB: Jurkat, Raji, HEK-293T, A431, HeLa, HepG2, H1299, HCT116, MCF-7, NT2D1, PC-3, U87-MG, NIH/3T3 whole cell lysate, mouse and rat brain tissue lysat, drosophila lysate. IHC: SAS xenograft tissue. IF: HeLa whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95966の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/20000. Predicted molecular weight: 50 kDa.
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ICC/IF |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/20000. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. -
組織特異性
Expressed at a high level in fetal brain. -
関連疾患
Lissencephaly 3 -
配列類似性
Belongs to the tubulin family. -
翻訳後修飾
Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling. -
細胞内局在
Cytoplasm, cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 7846 Human
- Entrez Gene: 22142 Mouse
- Entrez Gene: 64158 Rat
- Omim: 602529 Human
- SwissProt: Q71U36 Human
- SwissProt: P68369 Mouse
- SwissProt: P68370 Rat
- Unigene: 654422 Human
see all -
別名
- Alpha tubulin 3 antibody
- Alpha-tubulin 3 antibody
- B alpha 1 antibody
see all
画像
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All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7 : H1299 (human non-small cell lung carcinoma cell line) whole cell lysate
Lane 8 : HCT 116 (human colorectal carcinoma cell line) whole cell lysate
Lane 9 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 10 : NT2/D1 (human embryonal testis carcinoma cell line) whole cell lysate
Lane 11 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
Lane 12 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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Immunofluorescence analysis of 4% paraformaldehyde fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells lalbeling TUBA1A at cytoskeleton with ab95966 at 1/500 dilution. Blue: Hoechst 33342 staining.
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All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 2 : HEK-293T whole cell lysate at 5 µg
Lane 3 : HEK-293T whole cell lysate at 2 µg
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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Anti-TUBA1A antibody (ab95966) at 1/10000 dilution + Rat brain tissue lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
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Immunofluorescence analysis of 4% paraformaldehyde fixed, at RT for 15 min, HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling TUBA1A at cytoskeleton with ab95966 at 1/500 dilution. Blue: Hoechst 33342 staining.
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Anti-TUBA1A antibody (ab95966) at 1/10000 dilution + Drosophila lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 50 kDa10% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab95966 は論文での使用が確認できていません。