Anti-TUBA1 + TUBA3 + TUBA4 抗体 (ab110386)

製品の概要

  • 製品名Anti-TUBA1 + TUBA3 + TUBA4 antibody
    TUBA1 + TUBA3 + TUBA4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TUBA1 + TUBA3 + TUBA4
  • 特異性ab110386 detects endogenous levels of total TUBA1 + TUBA3 + TUBA4 protein.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Human
  • 免疫原

    A synthesized non-phosphopeptide derived from Human TUBA1 + TUBA3 + TUBA4 around the phosphorylation site of tyrosine 272 (A-T-YP-A-P).

  • ポジティブ・コントロール
    • Rat brain cells lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.88% Sodium chloride
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab110386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.

ターゲット情報

  • 機能Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • 組織特異性Expressed at a high level in fetal brain.
  • 関連疾患Defects in TUBA1A are the cause of lissencephaly type 3 (LIS3) [MIM:611603]. LIS is characterized by a smooth brain surface due to the absence (agyria) or reduction (pachygyria) of surface convolutions. It is often associated with psychomotor retardation and seizures. LIS3 features include agyria or pachygyria or laminar heterotopia, severe mental retardation, motor delay, variable presence of seizures, and abnormalities of corpus callosum, hippocampus, cerebellar vermis and brainstem.
  • 配列類似性Belongs to the tubulin family.
  • 翻訳後修飾Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha-tubulin 3 antibody
    • H2 ALPHA antibody
    • TBA1A_HUMAN antibody
    • TUBA1A antibody
    • TUBA3C antibody
    • TUBA3D antibody
    • TUBA3E antibody
    • Tubulin alpha 3E chain antibody
    • Tubulin alpha-1A chain antibody
    • Tubulin alpha-3 chain antibody
    • Tubulin B-alpha-1 antibody
    • Tubulin, alpha 1a antibody
    • Tubulin, alpha 1b antibody
    • Tubulin, alpha 1c antibody
    • Tubulin, alpha 3c antibody
    • Tubulin, alpha 3e antibody
    see all

Anti-TUBA1 + TUBA3 + TUBA4 antibody 画像

  • All lanes : Anti-TUBA1 + TUBA3 + TUBA4 antibody (ab110386) at 1/500 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat brain lysate with blocking peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa

Anti-TUBA1 + TUBA3 + TUBA4 antibody (ab110386) 使用論文

ab110386 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab110386.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"