The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Widely expressed with the highest levels observed in vascular tissues, lymph node, pituitary, lung and intestine. Not expressed in the liver.
Note=Defects in TTC37 are associated with Trico-Hepato-Enteric syndrome (THE). THE syndrome is a rare and severe autosonmal recessive condition that associates intractable diarrhea with facial dysmorphism, intrauterine growth retardation immunodeficiency with low serum concentrations of immunoglobulins and hair abnormalities characterized by woolly hair.