製品の概要

  • 製品名
    Anti-TTC36 antibody
  • 製品の詳細
    Rabbit polyclonal to TTC36
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    amino acids 2-64 (GTPNDQAVLQ AIFNPDTPFG DIVGLDLGEE AEKEEREEDE VFPQAQLEQS KALELQGVMA AEA) of Human TTC36.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Human liver tissue; Human liver lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab122507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment
IHC-P 1/900. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/250 - 1/500.

ターゲット情報

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  • Immunocytochemistry/ Immunofluorescence - Anti-TTC36 antibody (ab122507)
    Immunocytochemistry/ Immunofluorescence - Anti-TTC36 antibody (ab122507)
    Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122507 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Western blot - Anti-TTC36 antibody (ab122507)
    Western blot - Anti-TTC36 antibody (ab122507)
    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC36 antibody (ab122507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC36 antibody (ab122507)
    ab122507 at 1/900 dilution staining TTC36 in Paraffin-embedded Human Liver tissue by Immunohistochemistry.
  • Western blot - Anti-TTC36 antibody (ab122507)
    Western blot - Anti-TTC36 antibody (ab122507)
    All lanes : Anti-TTC36 antibody (ab122507) at 1/250 dilution

    Lane 1 : RT4 lysate
    Lane 2 : U251 MG lysate
    Lane 3 : Human plasma
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique.

参考文献

ab122507 has not yet been referenced specifically in any publications.

ab122507 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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