製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TTC19
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide: GTGAERLRTR GAPARGHGVL PLLAALAWFS RPAATAEQPG EDASDEAEAE, corresponding to N terminal amino acids 37-86 of Mouse TTC19

  • ポジティブ・コントロール
    • NIH3T3 lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab66622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay 1:62500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Mitochondrial protein required for formation of the mitochondrial complex III (PubMed:21278747). May also be required for the abcission step in cytokinesis, possibly regulating the ESCRT-III complex via its interaction with CHMP4B (PubMed:20208530). However, the involvement in cytokinesis requires additional experimental evidence.
  • 関連疾患
    Mitochondrial complex III deficiency, nuclear 2
  • 配列類似性
    Belongs to the TTC19 family.
    Contains 5 TPR repeats.
  • 細胞内局在
    Mitochondrion inner membrane. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Midbody. According to some authors, localizes to the centrosome during all stages of the cell cycle and is recruited to the midbody during cytokinesis (PubMed:20208530). However, the midbody localization could not be confirmed by others.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2010204O13Rik antibody
    • FLJ20343 antibody
    • MGC138312 antibody
    • MGC19520 antibody
    • mitochondrial antibody
    • RIKEN cDNA 2810460C24 antibody
    • Tetratricopeptide repeat protein 19 antibody
    • TPR repeat protein 19 antibody
    • Ttc 19 antibody
    • TTC19 antibody
    • TTC19_HUMAN antibody
    see all

画像

  • Anti-TTC19 antibody (ab66622) at 1.25 µg/ml + NIH3T3 lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 42 kDa
    Observed band size : 42 kDa

プロトコール

参考文献

ab66622 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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