製品の概要

  • 製品名Anti-TTC10 antibody
    TTC10 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to TTC10
  • 特異性This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2).
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Dog
  • 免疫原

    Synthetic peptide:

    C-KKRIDEDDFADEE

    , corresponding to C terminal amino acids 804-816 of Human TTC10

  • ポジティブ・コントロール
    • Human kidney tissue.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab42497 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution. PubMed: 19470799
IHC-P Use a concentration of 3 µg/ml.
Western Blot: Preliminary experiments in human kidney, liver and lung lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).

ターゲット情報

  • 機能Involved in primary cilium biogenesis.
  • 組織特異性Expressed in the heart, brain, liver, lung, kidney, skeletal muscle and pancreas.
  • 配列類似性Contains 12 TPR repeats.
  • 細胞内局在Cytoplasm > cytoskeleton > centrosome > centriole. Cytoplasm > cytoskeleton > cilium basal body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • D13S1056E antibody
    • DAF19 antibody
    • hTg737 antibody
    • Ift88 antibody
    • IFT88_HUMAN antibody
    • Intraflagellar transport 88 homolog antibody
    • Intraflagellar transport protein 88 homolog antibody
    • MGC26259 antibody
    • Polaris homolog antibody
    • Probe hTg737 (polycystic kidney disease, autosomal recessive) antibody
    • Recessive polycystic kidney disease protein Tg737 homolog antibody
    • RP11-172H24.2 antibody
    • Tetratricopeptide repeat domain 10 antibody
    • Tetratricopeptide repeat protein 10 antibody
    • TG737 antibody
    • TPR repeat protein 10 antibody
    • TTC10 antibody
    see all

Anti-TTC10 antibody 画像

  • ab42497 (3µg/ml) staining of paraffin embedded Human Kidney. The tissue sections were subjected to antigen retrieval by microwave in Tris/EDTA buffer. The HRP-staining procedure was used for detection.

  • ab42497 staining TTC10 in human prostate epithelial primary cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton ×100. Samples were incubated with primary antibody (1/100:in DMEM w/ 10% FBS) for 1 hour at 200C. An Alexa Fluor ® 546-conjugated donkey polyclonal to goat IgG was used undiluted as secondary antibody.

    See Abreview

Anti-TTC10 antibody (ab42497) 使用論文

This product has been referenced in:
  • Goggolidou P  et al. ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis. Development 141:3966-77 (2014). IF ; Mouse . Read more (PubMed: 25294941) »
  • Bodle JC  et al. Primary cilia: the chemical antenna regulating human adipose-derived stem cell osteogenesis. PLoS One 8:e62554 (2013). ICC/IF ; Human . Read more (PubMed: 23690943) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate epithelial - primary)
Specification Prostate epithelial - primary
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% triton x-100
Username

Dr. Luke Hofkamp

Verified customer

投稿 Feb 09 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Retrieval
Permeabilization Yes - Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Username

Abcam user community

Verified customer

投稿 Nov 20 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"