製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TTBK1
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within N terminal amino acids 240 - 270 of Human TTBK1

  • ポジティブ・コントロール
    • WB: Mouse testis tissue lysates IHC-P: brain tissue IF/ICC: SKNSH cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab103944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/100 - 1/500. Predicted molecular weight: 143 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Serine/threonine kinase which is able to phosphorylate TAU on serine, threonine and tyrosine residues. Induces aggregation of TAU.
  • 組織特異性
    Expressed in the brain, particularly in cortical and hippocampal neurons. Weakly expressed in spinal cord and testis. No expression in adipose tissue, bladder, cervix, colon, esophagus, heart, kidney, liver, lung, ovary, placenta, prostate, skeletal muscle, small intestine, spleen, testis, thymus, thyroid or trachea.
  • 配列類似性
    Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BDTK antibody
    • Brain derived tau kinase antibody
    • Brain-derived tau kinase antibody
    • KIAA1855 antibody
    • Tau tubulin kinase 1 antibody
    • Tau-tubulin kinase 1 antibody
    • TTBK1 antibody
    • TTBK1_HUMAN antibody
    see all

画像

  • ab103944 stained SKNSH cells. The cells were 4% formalehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab103944 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-TTBK1 antibody (ab103944) at 1/100 dilution + Mouse testis tissue lysates at 35 µg

    Predicted band size : 143 kDa
  • ab103944, at a 1/50 dilution, staining TTBK1 in formalin fixed and paraffin embedded brain tissue, followed by peroxidase conjugation of the secondary antibody and DAB staining.

参考文献

This product has been referenced in:
  • Liachko NF  et al. The tau tubulin kinases TTBK1/2 promote accumulation of pathological TDP-43. PLoS Genet 10:e1004803 (2014). WB . Read more (PubMed: 25473830) »

See 1 Publication for this product

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