製品の概要

  • 製品名Anti-TSG101 antibody [4A10]
    TSG101 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [4A10] to TSG101
  • 特異性This antibody recognizes the TSG-101 protein, the product of a recently identified tumor susceptibility gene the inactivation of which in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.
  • アプリケーション適用あり: Flow Cyt, ICC/IF, IP, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Drosophila melanogaster
  • 免疫原

    Fusion protein containing amino acids 167-374 of the TSG101 protein expressed in E. coli.

  • ポジティブ・コントロール
    • MOLT4, HeLa

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 23045692
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/100 - 1/1000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/100. Do not perform antigen retrieval.

ターゲット情報

  • 機能Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
  • 配列類似性Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
    Contains 1 SB (steadiness box) domain.
    Contains 1 UEV (ubiquitin E2 variant) domain.
  • ドメインThe UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
    The coiled coil domain may interact with stathmin.
    The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
  • 翻訳後修飾Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
  • 細胞内局在Cytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ESCRT I complex subunit TSG101 antibody
    • ESCRT-I complex subunit TSG101 antibody
    • TS101_HUMAN antibody
    • TSG 10 antibody
    • TSG 101 antibody
    • TSG10 antibody
    • Tsg101 antibody
    • Tumor susceptibility gene 10 antibody
    • Tumor susceptibility gene 101 antibody
    • Tumor susceptibility gene 101 protein antibody
    • Tumor susceptibility protein antibody
    • Tumor susceptibility protein isoform 3 antibody
    • VPS 23 antibody
    • VPS23 antibody
    see all

Anti-TSG101 antibody [4A10] 画像

  • All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1/500 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : HepG2 whole cell lysate
    Lane 4 : K562 whole cell lysate
    Lane 5 : THP-1 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 43 kDa
  • Immunohistochemical analysis of paraffin-embedded colon carcinoma tissue labeling TSG101 with ab83 at 1/100 dilution.

  • Immunofluorescence analysis of amyloid peptide-treated mouse astrocytes, staining TSG101 with ab83.

    Astrocytes were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100 for 5 min at room temperature. Cells were incubated with primary antibody and a fluorescent-conjugated anti-mouse IgG was used as the secondary antibody.
  • Panc-1 and Serum Exosomes were characterized by the exosome-specific expression of TSG101 by FACS analysis.

    Exosomes were attached to 4-μm aldehyde/sulfate latex beads by mixing ∼30 μg of exosomes in a 100-μl volume of beads for 2 hours at room temperature. This suspension was diluted to 1 ml with PBS, and the reaction was stopped with 100 mm glycine and 2% BSA in PBS. Exosome-bound beads were washed in PBS/1% BSA, blocked with 10% BSA, and stained for FACS with anti-TSG101 (1:400, ab83). An Alexa Fluor® 488 conjugated anti-mouse IgG was used as the secondary antibody.

  • All lanes : Anti-TSG101 antibody [4A10] (ab83) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : A431 whole cell lysate (ab7909)
    Lane 3 : Jurkat whole cell lysate (ab7899)
    Lane 4 : HEK293 whole cell lysate (ab7902)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Donkey polyclonal to Mouse IgG (IRDyeTM 700DX) at 1/10000 dilution

    Predicted band size : 43 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-TSG101 antibody [4A10] (ab83)

    Lane 1 : 20ug HeLa cell extract
    Lane 2 : 20ug HeLa cell extract from cells treated with TSG101 siRNA


    Predicted band size : 43 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)

Anti-TSG101 antibody [4A10] (ab83) 使用論文

This product has been referenced in:
  • Ruiz-Guillen M  et al. Capsid-deficient alphaviruses generate propagative infectious microvesicles at the plasma membrane. Cell Mol Life Sci 73:3897-916 (2016). Read more (PubMed: 27117550) »
  • Chakrabortty SK  et al. Extracellular vesicle-mediated transfer of processed and functional RNY5 RNA. RNA 21:1966-79 (2015). WB ; Human . Read more (PubMed: 26392588) »

See all 48 Publications for this product

Product Wall

Thank you for contacting us.

You can use any protease inhibitor, most common are Leupeptin and PMSF. These protease inhibitors should be dissolved in lysis buffer.

People tend to use protease inhibitors cocktails which have many en...

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Thank very much for filling the questionnaire and for sending the image.

I have reviewed the protocol now and would like to point out few things that may be the cause of multiple bands;

- Protease inhibitors; You have mentioned that...

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Thanks you for your email.

There could be many reasons for this extra band. It could be an Isoform; TSG101 have 2 isoforms one is 31kDa nd other is 44 kDa. Two bands could be observed however it will depend on the lysates being used. http://w...

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Thank you for contacting Abcam.

Forthese antibodies I would recommend using reducing conditions and also initially using a 1/1000 primary antibody dilution, although you may to alter this concentration for subsequent western blots.

...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Human HeLa)
Loading amount 30 µg
Specification Human HeLa
Gel Running Conditions Reduced Denaturing (12.5% gel)
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Nov 25 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - other (brain, HEK cell lysate)
Loading amount 50 µg
Specification brain, HEK cell lysate
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
Username

Dr. sriharsha kantamneni

Verified customer

投稿 Sep 26 2007

The datasheet was correct and the customer should have received 100ul, I apologise for this problem and have arranged a free of charge vial to be sent to you. Many thanks for your help with this matter,

Thank you for your email. I contacted the originator of this antibody who was able to provide me with the following information. "Concerning IHC staining, you may want to refer to the following publication for a detailed protocol: Zhu et at, Oncogene 2...

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Thank you for the details that you have provided and I'm sorry to hear that your customer is experiencing difficulty with this antibody. You mentioned that a previously purchased batch worked well; do you have that batch number? Were the protocols and ...

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For IF staining a dilution of 1:500 to 1:1000 should be sufficient to detect the protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"