Anti-TSEN34 抗体 (ab68868)
Key features and details
- Mouse polyclonal to TSEN34
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TSEN34 antibody
TSEN34 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to TSEN34 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human TSEN34. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- TSEN34 transfected 293T cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituents: 0.2% Monobasic dihydrogen potassium phosphate, 0.6% Dibasic monohydrogen sodium phosphate, 8% Sodium chloride, 0.2% Potassium chloride, 91% Water -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68868の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa). |
ターゲット情報
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機能
Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. It probably carries the active site for 3'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. -
関連疾患
Pontocerebellar hypoplasia 2C -
配列類似性
Belongs to the tRNA-intron endonuclease family. -
細胞内局在
Nucleus. Nucleus, nucleolus. May be transiently localized in the nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 79042 Human
- Omim: 608754 Human
- SwissProt: Q9BSV6 Human
- Unigene: 15580 Human
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別名
- HsSen34 antibody
- LENG 5 antibody
- LENG5 antibody
see all
画像
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All lanes : Anti-TSEN34 antibody (ab68868) at 1/500 dilution
Lane 1 : TSEN34 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 43 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (1)
ab68868 は 1 報の論文で使用されています。
- Breuss MW et al. Autosomal-Recessive Mutations in the tRNA Splicing Endonuclease Subunit TSEN15 Cause Pontocerebellar Hypoplasia and Progressive Microcephaly. Am J Hum Genet 99:228-35 (2016). PubMed: 27392077