製品の概要

  • 製品名Anti-TSEN2 antibody
    TSEN2 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to TSEN2
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human TSEN2 protein (NP_079541.1).

  • ポジティブ・コントロール
    • TSEN2 transfected 293T lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

ターゲット情報

  • 機能Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
  • 組織特異性Isoform 1 and isoform 2 are widely expressed at very low level.
  • 関連疾患Defects in TSEN2 are the cause of pontocerebellar hypoplasia type 2B (PCH2B) [MIM:612389]. Pontocerebellar hypoplasia (PCH) is a heterogeneous group of disorders characterized by an abnormally small cerebellum and brainstem. PCH type 2 is characterized by progressive microcephaly from birth combined with extrapyramidal dyskinesia and chorea, epilepsy, and normal spinal cord findings.
  • 配列類似性Belongs to the tRNA-intron endonuclease family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. Nucleus > nucleolus. May be transiently localized in the nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HsSen2 antibody
    • MGC2776 antibody
    • MGC4440 antibody
    • SEN2 antibody
    • SEN2_HUMAN antibody
    • SEN2L antibody
    • tRNA intron endonuclease Sen2 antibody
    • tRNA splicing endonuclease 2 homolog (SEN2, S. cerevisiae) antibody
    • tRNA splicing endonuclease subunit Sen2 antibody
    • tRNA-intron endonuclease sen2 antibody
    • tRNA-splicing endonuclease subunit Sen2 antibody
    • TSEN 2 antibody
    • TSEN2 antibody
    see all

Anti-TSEN2 antibody 画像

  • All lanes : Anti-TSEN2 antibody (ab69179) at 1/500 dilution

    Lane 1 : TSEN2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab69179 at 10µg/ml staining TSEN2 in human Hela cells by ICC/IF.

Anti-TSEN2 antibody (ab69179) 使用論文

ab69179 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"