The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
1/50 - 1/1000.
Use at an assay dependent concentration.
機能Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.
配列類似性Belongs to the class-I aminoacyl-tRNA synthetase family. Contains 1 WHEP-TRS domain.
翻訳後修飾Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS.
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Vellaichamy A et al. Proteomic interrogation of androgen action in prostate cancer cells reveals roles of aminoacyl tRNA synthetases. PLoS One4:e7075 (2009).
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