Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase
Mouse, Rat, Human
A synthetic peptide corresponding to residues near the C-terminus of human Tryptophan Hydroxylase
THP-1 cell lysate and human urinary bladder carcinoma.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/50 - 1/100.
Isoform 2 seems to be less widely expressed than isoform 1.
Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
Contains 1 ACT domain.
Information by UniProt
Indoleacetic acid 5 hydroxylase antibody
L tryptophan hydroxylase antibody
Western blot - Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954)
Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954) at 1/500 dilution + THP-1 cell lysate at 10 µg
Secondary goat anti-rabbit HRP at 1/2000 dilution Predicted band size: 51 kDa Observed band size: 51 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954)
ab52954 at 1/50 dilution staining Tryptophan Hydroxylase in human urinary bladder carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Flow Cytometry - Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954)
Overlay histogram showing HeLa cells stained with ab52954 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52954, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (
) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10
cells) used under the same conditions. Acquisition of >5,000 events was performed.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"