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RabMAb

Anti-Tryptophan Hydroxylase 抗体 [EP1311Y] (ab52954)

製品の概要

  • 製品名
    Anti-Tryptophan Hydroxylase antibody [EP1311Y]
    Tryptophan Hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1311Y] to Tryptophan Hydroxylase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues near the C-terminus of human Tryptophan Hydroxylase

  • ポジティブ・コントロール
    • THP-1 cell lysate and human urinary bladder carcinoma.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IP 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.

ターゲット情報

  • 組織特異性
    Isoform 2 seems to be less widely expressed than isoform 1.
  • パスウェイ
    Aromatic compound metabolism; serotonin biosynthesis; serotonin from L-tryptophan: step 1/2.
  • 配列類似性
    Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
    Contains 1 ACT domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Indoleacetic acid 5 hydroxylase antibody
    • L tryptophan hydroxylase antibody
    • MGC119994 antibody
    • TPH 1 antibody
    • TPH antibody
    • TPH1 antibody
    • TPH1_HUMAN antibody
    • TPRH antibody
    • TRPH antibody
    • Tryptophan 5 hydroxylase 1 antibody
    • Tryptophan 5 monooxygenase 1 antibody
    • Tryptophan 5 monooxygenase antibody
    • Tryptophan 5-hydroxylase 1 antibody
    • Tryptophan 5-monooxygenase 1 antibody
    • Tryptophan hydroxylase 1 antibody
    see all

画像

  • Anti-Tryptophan Hydroxylase antibody [EP1311Y] (ab52954) at 1/500 dilution + THP-1 cell lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa

  • ab52954 at 1/50 dilution staining Tryptophan Hydroxylase in human urinary bladder carcinoma by Immunohistochemistry, Paraffin embedded tissue.
  • Overlay histogram showing HeLa cells stained with ab52954 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52954, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

参考文献

This product has been referenced in:
  • Zhu X  et al. Nicotinamide adenine dinucleotide replenishment rescues colon degeneration in aged mice. Signal Transduct Target Ther 2:17017 (2017). Read more (PubMed: 29263919) »
  • Acosta-Andrade C  et al. A novel role for antizyme inhibitor 2 as a regulator of serotonin and histamine biosynthesis and content in mouse mast cells. Amino Acids 48:2411-21 (2016). Read more (PubMed: 27084713) »

See all 10 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

Filter by Ratings

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 20 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Brain
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

投稿 Jan 08 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, Raphe Nucleus)
Specification
Brain, Raphe Nucleus
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 0.1%
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 26 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Rat Tissue sections (Hindbrain)
Specification
Hindbrain
Username

Abcam user community

Verified customer

投稿 Apr 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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