Anti-Trypanosoma brucei phosphoglycerate kinase 抗体 (ab89651)

製品の概要

  • 製品名
    Anti-Trypanosoma brucei phosphoglycerate kinase antibody
  • 製品の詳細
    Rabbit polyclonal to Trypanosoma brucei phosphoglycerate kinase
  • アプリケーション
    適用あり: ICC/IF, ICC, WBmore details
  • 種交差性
    Reacts with Trypanosoma brucei
  • 免疫原

    Synthetic peptide derived from the C terminal domain of Trypanosoma brucei phosphoglycerate kinase.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent dilution.
ICC Use at an assay dependent concentration.
WB 1/200 - 1/2000. Predicted molecular weight: 55 kDa.

ターゲット情報

  • 関連性
    Belongs to the phosphoglycerate kinase family.
  • 別名
    • PGK A allele 2 antibody
    • Phosphoglycerate kinase A antibody
    • Trypanosoma brucei PGK antibody

画像

  • All lanes : Anti-Trypanosoma brucei phosphoglycerate kinase antibody (ab89651) at 1/200 dilution (in TBS + 5% milk for 12 hours at 4°C)

    Lane 1 : Whole cell lysate of Trypanosoma brucei - procyclic form
    Lane 2 : Whole cell lysate of Trypanosoma brucei - bloodstream

    Lysates/proteins at 20000000 cells per lane.

    Secondary
    A biotin-conjugated Goat anti-rabbit IgG polyclonal at 1/200 dilution

    Predicted band size : 55 kDa
    Observed band size : 65,75 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Susan Rouse

    See Abreview

参考文献

ab89651 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Sample
Trypanosoma brucei Cell lysate - whole cell (Trypanosoma brucei procyclic form and bloodstream)
Loading amount
2e+007 cells
Specification
Trypanosoma brucei procyclic form and bloodstream
Gel Running Conditions
Reduced Denaturing (10% separating 4% stacking gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Susan Rouse

Verified customer

投稿 Aug 26 2010

Application
Immunocytochemistry
Sample
Trypanosoma brucei Cultured Cells (Blood stream and procyclic forms)
Specification
Blood stream and procyclic forms
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5% · Temperature: 37°C
Username

Dr. Susan Rouse

Verified customer

投稿 Aug 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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