製品の概要

  • 製品名Anti-TRUB1 antibody
    TRUB1 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to TRUB1
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human protein (NP_631908.1)

  • ポジティブ・コントロール
    • WB: mammalian transfected lysate. ICC/IF: HeLa cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 37 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能May be responsible for synthesis of pseudouridine from uracil in transfer RNAs.
  • 組織特異性Highly expressed in heart, skeletal muscle and liver. Expressed at lower levels in lung, small intestine, kidney and spleen.
  • 配列類似性Belongs to the pseudouridine synthase TruB family.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Probable tRNA pseudouridine synthase 1 antibody
    • PUS4 antibody
    • TruB pseudouridine (psi) synthase homolog 1 (E. coli) antibody
    • Trub1 antibody
    • TRUB1_HUMAN antibody
    see all

Anti-TRUB1 antibody 画像

  • All lanes : Anti-TRUB1 antibody (ab72866) at 1/500 dilution

    Lane 1 : TRUB1-transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 37 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells using protein A affiinity purified ab72866 TRUB1 at 10 ug/ml.

Anti-TRUB1 antibody (ab72866) 使用論文

ab72866 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry regarding ab72866. I can confirm that this antibody is affinity purified and we have just removed the purification note in the application section to avoid any further confusion. It has only been tested for Western bl...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"