製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRPV4
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human, Pig
    交差が予測される動物種: Mouse, Rat, Rabbit, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 720-769 (

    VDEVNWSHWNQNLGIINEDPGKNETYQYYGFSHTVGRLRRDRWSSVVPRV

    ) of Human TRPV4 isoform 2 (NP_671737)

  • ポジティブ・コントロール
    • HT1080 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 91 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
  • 組織特異性
    Found in the synoviocytes from patients with (RA) and without (CTR) rheumatoid arthritis (at protein level).
  • 関連疾患
    Brachyolmia 3
    Spondylometaphyseal dysplasia Kozlowski type
    Metatropic dysplasia
    Distal spinal muscular atrophy, congenital non-progressive
    Charcot-Marie-Tooth disease 2C
    Scapuloperoneal spinal muscular atrophy
    Spondyloepiphyseal dysplasia Maroteaux type
    Parastremmatic dwarfism
    Digital arthropathy-brachydactyly, familial
  • 配列類似性
    Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV4 sub-subfamily.
    Contains 3 ANK repeats.
  • 翻訳後修飾
    Phosphorylation results in enhancement of its channel function.
  • 細胞内局在
    Cell membrane and Cell membrane. Cell junction > adherens junction. Assembly of the putative homotetramer occurs primarily in the endoplasmic reticulum.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BCYM3 antibody
    • CMT2C antibody
    • HMSN2C antibody
    • osm 9 like TRP channel 4 antibody
    • Osm-9-like TRP channel 4 antibody
    • OSM9 like transient receptor potential channel 4 antibody
    • Osmosensitive transient receptor potential channel 4 antibody
    • OTRPC 4 antibody
    • OTRPC4 antibody
    • SMAL antibody
    • SPSMA antibody
    • SSQTL1 antibody
    • Transient receptor potential cation channel subfamily V member 4 antibody
    • Transient receptor potential protein 12 antibody
    • TRP 12 antibody
    • TRP12 antibody
    • TRPV 4 antibody
    • TrpV4 antibody
    • TRPV4_HUMAN antibody
    • Vanilloid receptor like channel 2 antibody
    • Vanilloid receptor like protein 2 antibody
    • Vanilloid receptor related osmotically activated channel antibody
    • Vanilloid receptor-like channel 2 antibody
    • Vanilloid receptor-like protein 2 antibody
    • Vanilloid receptor-related osmotically-activated channel antibody
    • VR 4 antibody
    • VR OAC antibody
    • VR-OAC antibody
    • VR4 antibody
    • VRL 2 antibody
    • VRL-2 antibody
    • VRL2 antibody
    • VROAC antibody
    see all

画像



  • Predicted band size : 91 kDa
    Western blot analysis of PANC1 cells labeling TRPV4 with ab94868 at 1.0µg/ml.
  • ab94868 staining TRPV4 in cultured hippocampal slices of rat brain by Immunohistochemistry - PFA fixed sections.

    Slice cultures attached to the PTFE membrane were fixed with 4% paraformaldehyde in PBS for 20 minutes at room temperature. They were permeabilized with 1% Triton X-100 in PBS overnight at 4 °C, then blocked with 3% bovine serum albumin (BSA) in PBS and incubated overnight at 4 °C with ab94868 (Red) at a 1/500 dilution. Following washes with PBS and blocking with 3% BSA, tissue was incubated for 1 hour at room temperature with Alexa Fluor 568-conjugated goat anti-rabbit antibody diluted 1/500 in blocking solution. Tissue was washed in PBS, mounted with DAPI.

    Blue: DAPI.
    Green: anti-Map2.
    Red: ab94868.
  • Anti-TRPV4 antibody (ab94868) at 1 µg/ml + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 91 kDa
    Gel concentration: 6-18%

参考文献

This product has been referenced in:
  • Alkozi HA  et al. TRPV4 Stimulation Induced Melatonin Secretion by Increasing Arylalkymine N-acetyltransferase (AANAT) Protein Level. Int J Mol Sci 18:N/A (2017). IHC-Fr ; Human . Read more (PubMed: 28368307) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (Bio-Rad 4–20% Mini-PROTEAN­ TGX™ Gel, 12 well, 20 ´l 456-1095)
Sample
Pig Tissue lysate - whole (coronary artery)
Specification
coronary artery
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Aug 29 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録