製品の概要

  • 製品名Anti-TRPM8 antibody [EPR4196(2)]
    TRPM8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4196(2)] to TRPM8
  • アプリケーション適用あり: ICC, WBmore details
    適用なし: Flow Cyt,IHC-P or IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in the extracellular domain of Human TRPM8.

  • ポジティブ・コントロール
    • HT-1376, LnCaP and A549 cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC 1/250 - 1/500.
WB 1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 128 kDa).Can be blocked with TRPM8 peptide (ab187961).
  • 追加情報Is unsuitable for Flow Cyt,IHC-P or IP.
  • ターゲット情報

    • 機能Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
    • 組織特異性Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
    • 配列類似性Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Long transient receptor potential channel 6 antibody
      • LTrpC-6 antibody
      • LTrpC6 antibody
      • MGC2849 antibody
      • Short form of the TRPM8 cationic channel antibody
      • Transient receptor potential cation channel subfamily M member 8 antibody
      • Transient receptor potential p8 antibody
      • transient receptor potential-p8 antibody
      • Trp p8 antibody
      • Trp-p8 antibody
      • Trpm8 antibody
      • TRPM8_HUMAN antibody
      • TRPP8 antibody
      see all

    Anti-TRPM8 antibody [EPR4196(2)] 画像

    • All lanes : Anti-TRPM8 antibody [EPR4196(2)] (ab109308) at 1/1000 dilution

      Lane 1 : HT-1376 cell lysates
      Lane 2 : LnCaP cell lysates
      Lane 3 : A549 cell lysates

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 128 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)

    Anti-TRPM8 antibody [EPR4196(2)] (ab109308) 使用論文

    This product has been referenced in:
    • Borowiec AS  et al. Cold/menthol TRPM8 receptors initiate the cold-shock response and protect germ cells from cold-shock-induced oxidation. FASEB J N/A:N/A (2016). Read more (PubMed: 27317670) »
    • Winking M  et al. Importance of a conserved sequence motif in transmembrane segment S3 for the gating of human TRPM8 and TRPM2. PLoS One 7:e49877 (2012). WB ; Human . Read more (PubMed: 23185472) »

    See all 2 Publications for this product

    Product Wall

    The immunogen sequence for this antibody is CVELDEHNLPRFPEW and range is 940-954.

    Thank you for contacting us.

    Yes, we recommend running the WB in reduced and denatured conditions.

    You can find the recommended protocol here: http://www.epitomics.com/products/support/wb

    I hope this information is helpf...

    Read More

    Thank you for contacting Abcam.

    We spoke earlier about two antibodies:
    anti-BMPR2 ab78422 (http://www.abcam.com/bmpr2-antibody-mm0060-9a10-ab78422.html)
    anti-TRPM8 ab109308 (http://www.abcam.com/trpm8-antibody-epr41962-ab109308.html) Read More

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Prostate)
    Specification Prostate
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization Yes - Tween 0.1%
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 1.2% · Temperature: 37°C
    Username

    Dr. Gabriel Bidaux

    Verified customer

    投稿 Aug 16 2012

    Merci de votre intérêt pour les anticorps Abcam anti-TRPM8.

    Comme expliqué par téléphone,certains anti-TRPM8 n’ont pas encore été testés chez la souris et nous n’offrons pas d...

    Read More

    Thank you for your interest in these antibodies. Without a discount the prices range from €360.00 to €370.00. A bulk order discount of approximately 5% is available for orders of 5 units or more of 5 different antibodies. The discount my collea...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"