製品の概要

  • 製品名Anti-TRPM8 antibody
    TRPM8 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRPM8
  • 特異性Reacts with residues 278-292 and the C terminus sequence of the human trpm8 protein.
  • アプリケーション適用あり: ELISA, IP, IHC-P, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Guinea pig, Human
  • 免疫原

    Synthetic peptide corresponding to Human TRPM8.
    Sequence:

    RNQLEKYISERTIQD Plus NDLKGLLKEIANKIK

  • ポジティブ・コントロール
    • Transfected Cos cell lysate. PMID 17510704, prostate cells used as positive control. PC-3 suitable negative control.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100 - 1/2000.
IP Use at an assay dependent concentration.

used at 6 ug/mg cell lysate.

IHC-P 1/10 - 1/2000.
WB 1/500 - 1/1000. Predicted molecular weight: 128 kDa.
IHC-Fr 1/100. See Abreview.

ターゲット情報

  • 機能Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • 組織特異性Expressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • 配列類似性Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Long transient receptor potential channel 6 antibody
    • LTrpC-6 antibody
    • LTrpC6 antibody
    • MGC2849 antibody
    • Short form of the TRPM8 cationic channel antibody
    • Transient receptor potential cation channel subfamily M member 8 antibody
    • Transient receptor potential p8 antibody
    • transient receptor potential-p8 antibody
    • Trp p8 antibody
    • Trp-p8 antibody
    • Trpm8 antibody
    • TRPM8_HUMAN antibody
    • TRPP8 antibody
    see all

Anti-TRPM8 antibody 画像

  • Anti-TRPM8 antibody (ab3243) at 1/750 dilution + TRPM8 transfected Cos7 cell lysate

    Predicted band size : 128 kDa
  • ab3243  staining TRPM8 in human prostate cancer tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • ab3243 staining TRPM8 in murine skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized with 0.4% Triton X-100, blocked using 10% serum for 30 minutes at 25°C and then incubated with ab3243 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

Anti-TRPM8 antibody (ab3243) 使用論文

This product has been referenced in:
  • Gardiner JC  et al. The role of TRPM8 in the Guinea-pig bladder-cooling reflex investigated using a novel TRPM8 antagonist. Eur J Pharmacol 740:398-409 (2014). IHC-P ; Guinea pig . Read more (PubMed: 25058909) »
  • Denda M  et al. Topical application of TRPM8 agonists accelerates skin permeability barrier recovery and reduces epidermal proliferation induced by barrier insult: role of cold-sensitive TRP receptors in epidermal permeability barrier homoeostasis. Exp Dermatol 19:791-5 (2010). IHC-P ; Mouse . Read more (PubMed: 20636355) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Immuno-precipitation step Protein A
Sample Human Cell lysate - whole cell (Hek cells transfected with human TRPM8)
Specification Hek cells transfected with human TRPM8
Total protein in input 800 µg
Username

Miss. Larisa Maier

Verified customer

投稿 Sep 17 2013

Gracias por contactarnos, le pido disculpas por la espera.

Adjunto al email la datasheet del producto descatalogado ab3243. Al no estar disponible no tengo acceso a la datasheet en formato pdf, por lo que la envío copiada en un documen...

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Thank you for contacting Abcam. Please find the protocol used to test ab3243 in IHC below. If there is anything else I can help you with, please let me know.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (skin, HR-1)
Specification skin, HR-1
Fixative Methanol
Permeabilization Yes - 0.4% TritonX100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 20 2011

Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab3243 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the e...

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Thank you for your e-mail to Hugh who is away today. I'm very sorry to hear you are still experiencing lots of non specific bands with ab3243 even after the protocol modifications. I would be happy to offer you a replacement vial or refund if you p...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionaire and I have a few comments. We have details of this antibody applied by IHC from customer feedback,...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (prostate cancer)
Specification prostate cancer
Blocking step Other as blocking agent for 30 minute(s) · Concentration: 1.2%
Username

Dr. Gabriel Bidaux

Verified customer

投稿 Aug 21 2006

Thank you for your enquiry. I am sorry but we do not have the immunogen peptide for this antibody in our catalogue. It may have been exhausted. Yes, were you to have the two peptides used to raise this antibody synthesized I can see no reason why t...

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Thank you for your patience and for providing some very useful and important details of your Western blot application and your experimental conditions. Looking at our record, I can clearly see that we have 3 complaints about the batch you received ...

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