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Fusion protein: LKLPDLKRND YTPDKIIFPQ DESSDLNLQS GNSTKESEAT NSVRLML, corresponding to cytoplasmic C terminal amino acids 1817-1863 of mouse TRPM7.
Our Abpromise guarantee covers the use of ab85016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 212 kDa. Prepare membrane fraction and ensure proteolysis is avoided. After extraction use an SDS buffer, incubate for 15 mins at 37°C before running on SDS-Page.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab85016 staining TRPM7 in human hippocampus tissue section by IHC-P. Bouin's fixed and paraffin-embedded tissue sections were used.