製品の概要

  • 製品名Anti-TRPM4 antibody [10H5]
    TRPM4 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [10H5] to TRPM4
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Dog, Human, Monkey
  • 免疫原

    Recombinant full length Human TRPM4 produced in HEK293T cells (NP_060106).

  • ポジティブ・コントロール
    • HEK293T cells transfected with pCMV6-ENTRY TRPM4 cDNA; HepG2, HeLa, HT29, COS7, Jurkat, MDCK, PC12, MCF7 cell extracts; Human Kidney, Liver, Liver carcinoma, Ovary and Pancreas tissues.
  • 特記事項Dilute in PBS (pH7.3) before use.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab123936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000 - 1/10000. Predicted molecular weight: 134 kDa.
IHC-P 1/50.

ターゲット情報

  • 機能Calcium-activated non selective (CAN) cation channel that mediates membrane depolarization. While it is activated by increase in intracellular Ca(2+), it is impermeable to it. Mediates transport of monovalent cations (Na(+) > K(+) > Cs(+) > Li(+)), leading to depolarize the membrane. It thereby plays a central role in cadiomyocytes, neurons from entorhinal cortex, dorsal root and vomeronasal neurons, endocrine pancreas cells, kidney epithelial cells, cochlea hair cells etc. Participates in T-cell activation by modulating Ca(2+) oscillations after T lymphocyte activation, which is required for NFAT-dependent IL2 production. Involved in myogenic constriction of cerebral arteries. Controls insulin secretion in pancreatic beta-cells. May also be involved in pacemaking or could cause irregular electrical activity under conditions of Ca(2+) overload. Affects T-helper 1 (Th1) and T-helper 2 (Th2) cell motility and cytokine production through differential regulation of calcium signaling and NFATC1 localization. Enhances cell proliferation through up-regulation of the beta-catenin signaling pathway.
  • 組織特異性Widely expressed with a high expression in intestine and prostate. In brain, it is both expressed in whole cerebral arteries and isolated vascular smooth muscle cells. Prominently expressed in Purkinje fibers. Expressed at higher levels in T-helper 2 (Th2) cells as compared to T-helper 1 (Th1) cells.
  • 関連疾患Defects in TRPM4 are the cause of progressive familial heart block type 1B (PFHB1B) [MIM:604559]. It is a cardiac bundle branch disorder characterized by progressive alteration of cardiac conduction through the His-Purkinje system, with a pattern of a right bundle-branch block and/or left anterior hemiblock occurring individually or together. It leads to complete atrio-ventricular block causing syncope and sudden death.
  • 配列類似性Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM4 sub-subfamily.
  • 翻訳後修飾Phosphorylation by PKC leads to increase the sensitivity to Ca(2+).
    Sumoylated. Desumoylated by SENP1.
  • 細胞内局在Endoplasmic reticulum. Golgi apparatus and Cell membrane. Endoplasmic reticulum. Golgi apparatus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110030C19Rik antibody
    • AW047689 antibody
    • Calcium-activated non-selective cation channel 1 antibody
    • FLJ20041 antibody
    • hTRPM4 antibody
    • Long transient receptor potential channel 4 antibody
    • LTrpC-4 antibody
    • LTrpC4 antibody
    • Melastatin 4 antibody
    • Melastatin like 2 protein antibody
    • Melastatin-4 antibody
    • Melastatin-like 2 antibody
    • Mls2s antibody
    • PFHB1B antibody
    • Transient receptor potential cation channel subfamily M member 4 antibody
    • Transient receptor potential cation channel, subfamily M, member 4 antibody
    • Trpm4 antibody
    • TRPM4_HUMAN antibody
    • TRPM4B antibody
    see all

Anti-TRPM4 antibody [10H5] 画像

  • All lanes : Anti-TRPM4 antibody [10H5] (ab123936) at 1/5000 dilution

    Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T cells transfected with pCMV6-ENTRY TRPM4 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 134 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216888)
  • All lanes : Anti-TRPM4 antibody [10H5] (ab123936) at 1/5000 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HeLa cell extract
    Lane 3 : HT29 cell extract
    Lane 4 : A549 cell extract
    Lane 5 : COS7 cell extract
    Lane 6 : Jurkat cell extract
    Lane 7 : MDCK cell extract
    Lane 8 : PC12 cell extract
    Lane 9 : MCF7 cell extract

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 134 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC216888)
  • ab123936 at 1/50 dilution staining TRPM4 in Paraffin-embedded Human Kidney tissue by Immunohistochemistry.
  • ab123936 at 1/50 dilution staining TRPM4 in Paraffin-embedded Human liver tissue by Immunohistochemistry.
  • ab123936 at 1/50 dilution staining TRPM4 in Paraffin-embedded Human liver carcinoma tissue by Immunohistochemistry.
  • ab123936 at 1/50 dilution staining TRPM4 in Paraffin-embedded Human Ovary tissue by Immunohistochemistry.
  • ab123936 at 1/50 dilution staining TRPM4 in Paraffin-embedded Human Pancreas tissue by Immunohistochemistry.

Anti-TRPM4 antibody [10H5] (ab123936) 使用論文

ab123936 has not yet been referenced specifically in any publications.

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