The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/312500. This antibody has been tested against the recombinant peptide.
Use at an assay dependent concentration.
Use a concentration of 1.25 µg/ml. Predicted molecular weight: 198 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Transient receptor potential cation channel, subfamily M, member 3 (TRPM3) belongs to the family of transient receptor potential (TRP) channels. TRP channels are cation selective channels important for cellular calcium signaling and homeostasis. TRPM3 mediates calcium entry, and this entry is potentiated by calcium store depletion. Its activity is increased by reduction in extracellular osmolarity, by store depletion and muscarinic receptor activation. The TRPM3-mediated calcium entry is inhibited by the nonselective calcium channel blocker lanthanide gadolinium. Expressed primarily in the kidney and, at lower levels, in brain, testis, ovary, pancreas and spinal cord. In the kidney, expressed predominantly in the collecting tubular epithelium in the medulla, medullary rays, and periglomerular regions; in the brain, highest levels are found in the cerebellum, choroid plexus, the locus coeruleus, the posterior thalamus and the substantia nigra. Down-regulated in renal tumors compared to normal kidney. Alternatively spliced transcript variants encoding different isoforms have been identified.
long transient receptor potential channel 3 antibody
LTrpC 3 antibody
melastatin 2 antibody
transient receptor potential cation channel, subfamily M, member 3 antibody
Western blot - TRPM3 antibody (ab56171)
Anti-TRPM3 antibody (ab56171) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
Secondary HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 198 kDa
Immunocytochemistry/ Immunofluorescence - TRPM3 antibody (ab56171)This image is a courtesy of an anonymous Abreview.
ab56171 staining TRPM3 in Rat murine choroid plexus cells by Immunocytochemistry/Immunofluorescence. Cells were fixed in PFA and permeabilized in Triton X-100 prior to blocking in 0.5% BSA in TBS-Tween for 20 minutes at room temperature. The primary antibody was diluted 1/100 and incubated with the sample for 16 hours at 4°C. The secondary antibody was Cy3®-conjugated Goat anti-Rabbit IgG (H+L) polyclonal, diluted 1/400. Nuclei counterstained with DAPI.