Anti-TRPC6 抗体 (ab62461)
Key features and details
- Rabbit polyclonal to TRPC6
- Suitable for: WB, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-TRPC6 antibody
TRPC6 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to TRPC6 -
由来種
Rabbit -
特異性
This antibody does potentially cross-react with TRPC3. -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human TRPC6 (N terminal).
Database link: Q9Y210 -
ポジティブ・コントロール
- WB: Human K562 cell lysate. IHC-P: Mouse lung tissue. ICC: K562 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62461の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).
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ICC |
Use a concentration of 2 - 5 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa). |
ICC
Use a concentration of 2 - 5 µg/ml. |
ターゲット情報
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機能
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion. -
組織特異性
Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm. -
関連疾患
Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. -
配列類似性
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
Contains 4 ANK repeats. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7225 Human
- Omim: 603652 Human
- SwissProt: Q9Y210 Human
- Unigene: 159003 Human
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別名
- bZ1P14.9 antibody
- FLJ11098 antibody
- FLJ14863 antibody
see all
画像
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Lane 1 : Anti-TRPC6 antibody (ab62461) at 0.5 µg/ml
Lane 2 : Anti-TRPC6 antibody (ab62461) at 1 µg/ml
All lanes : K562 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa -
ICC analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 (red) using ab62461 (5 µg/ml).
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ICC analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells stained for TRPC6 using ab62461 (2 µg/ml).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (20)
ab62461 は 20 報の論文で使用されています。
- Wang L et al. Role of TRPC6 in periodontal tissue reconstruction mediated by appropriate stress. Stem Cell Res Ther 13:401 (2022). PubMed: 35932080
- Chen PH et al. Lithium Reduces Migration and Collagen Synthesis Activity in Human Cardiac Fibroblasts by Inhibiting Store-Operated Ca2+ Entry. Int J Mol Sci 22:N/A (2021). PubMed: 33467715
- Zang Y et al. Astragaloside IV inhibits palmitic acid-induced apoptosis through regulation of calcium homeostasis in mice podocytes. Mol Biol Rep 48:1453-1464 (2021). PubMed: 33606151
- Qin Y et al. Overexpressed lncRNA AC068039.4 Contributes to Proliferation and Cell Cycle Progression of Pulmonary Artery Smooth Muscle Cells Via Sponging miR-26a-5p/TRPC6 in Hypoxic Pulmonary Arterial Hypertension. Shock 55:244-255 (2021). PubMed: 33026218
- Peng W et al. Hsa_circ_0003204 Knockdown Weakens Ox-LDL-Induced Cell Injury by Regulating miR-188-3p/TRPC6 Axis in Human Carotid Artery Endothelial Cells and THP-1 Cells. Front Cardiovasc Med 8:731890 (2021). PubMed: 34912856