製品の概要

  • 製品名Anti-TRPC6 antibody
    TRPC6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRPC6
  • アプリケーション適用あり: IHC-FoFr, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Human
  • 免疫原

    Synthetic peptide corresponding to Mouse TRPC6 aa 24-38 (N terminal).
    Sequence:

    CRRNESQDYLLMDELG


    Database link: Q61143

  • ポジティブ・コントロール
    • Rat brain.
  • 特記事項If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr Use at an assay dependent concentration.
WB 1/200 - 1/400. Predicted molecular weight: 111 kDa.

ターゲット情報

  • 機能Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • 組織特異性Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • 関連疾患Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • 配列類似性Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all

Anti-TRPC6 antibody 画像



  • Predicted band size : 111 kDa

    Lane 1:Anti-TRPC6 antibody, (1:200).

    Lane 2: Anti-TRPC6 antibody, preincubated with the control peptide antigen. 

  • ab12249 staining TRPC6 in mouse Olfactory tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Mice were anesthetized with 20% chloral hydrate (2 mg/g body weight), perfused transcardially with 0.9% saline followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB). The olfactory organs were dissected and postfixed in the same fixative for 15 min to overnight. Cryosections were rinsed in 0.1 M phosphate buffered saline (PBS), blocked in blocking solution containing 1% BSA, 3% normal donkey serum, and 0.3% Triton X-100 in PBS for 2 hours, and then incubated in the primary antisera overnight to 2 days. An Alexa Fluor® 568 conjugated anti rabbit IgG was used for 2 hours as secondary. ab12249 stains red in image shown.

Anti-TRPC6 antibody (ab12249) 使用論文

This product has been referenced in:
  • Letavernier E  et al. Williams-Beuren syndrome hypercalcemia: is TRPC3 a novel mediator in calcium homeostasis? Pediatrics 129:e1626-30 (2012). ICC/IF, IHC ; Human . Read more (PubMed: 22566418) »
  • Li W  et al. Activity-dependent BDNF release and TRPC signaling is impaired in hippocampal neurons of Mecp2 mutant mice. Proc Natl Acad Sci U S A 109:17087-92 (2012). WB ; Mouse . Read more (PubMed: 23027959) »

See all 4 Publications for this product

Product Wall

Thank you for your email. With ab12249 you should see a band at approximately 111 kDa. It sounds that perhaps there is an unreported novel protein that shares a similar epitope and could possibly be from the same protein family that is being detected. ...

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Thank you for your email. The free of charge replacement vial is being sent to your attention on order reference # 109650. The item is currently out of stock and you should receive the replacement early next week. You will receive emails regarding ship...

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Thank you for the details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab12249. This antibody should recognize the TRPC6 protein from rat brain and I do not see any problems with your protocol. I therefore can ...

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Thank you for your enquiry. I would suggest using rat brain as a positive control for this antibody. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"