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CRRNESQDYLLMDELG, corresponding to N-term amino acids 24-38 of Mouse TRPC6.
Our Abpromise guarantee covers the use of ab12249 in the following tested applications.
Lane 1:Anti-TRPC6 antibody, (1:200).
Lane 2: Anti-TRPC6 antibody, preincubated with the control peptide antigen.
ab12249 staining TRPC6 in mouse Olfactory tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Mice were anesthetized with 20% chloral hydrate (2 mg/g body weight), perfused transcardially with 0.9% saline followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB). The olfactory organs were dissected and postfixed in the same fixative for 15 min to overnight. Cryosections were rinsed in 0.1 M phosphate buffered saline (PBS), blocked in blocking solution containing 1% BSA, 3% normal donkey serum, and 0.3% Triton X-100 in PBS for 2 hours, and then incubated in the primary antisera overnight to 2 days. An Alexa Fluor® 568 conjugated anti rabbit IgG was used for 2 hours as secondary. ab12249 stains red in image shown.