製品の概要

  • 製品名Anti-TRPC3 antibody
    TRPC3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRPC3
  • 特異性ab51560 can be used for the detection of TRPC3.
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide, corresponding to 14 amino acids near to the N terminal of Human TRPC3

  • ポジティブ・コントロール
    • Mouse heart tissue and lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 97 kDa).Can be blocked with TRPC3 peptide (ab75651).

ターゲット情報

  • 関連性TRPC channels form the subfamily of channels in human most closely related to drosophila TRP channels. Structurally, this family shares a number of similar characteristics. At the proximal C-terminus of this sub-family is a TRP box motif containing the invariant EWKFAR sequence and between 3 and 4 ankyrin repeats near the N-terminus. These channels are non-selectively permeable to cations, with a selectivity of calcium over sodium variable among the different members. Many of TRPC channel subunits are able to coassemble.
  • 細胞内局在Cell Membrane; multi-pass membrane protein
  • 参照データベース
  • 別名
    • Htrp 3 antibody
    • Htrp3 antibody
    • MGC124333 antibody
    • Mtrp3 antibody
    • Receptor activated cation channel TRP3 antibody
    • Short transient receptor potential channel 3 antibody
    • Transient receptor potential cation channel subfamily C member 3 antibody
    • transient receptor potential cation channel subfamily C member 3 variant c antibody
    • Transient receptor potential channel 3 antibody
    • TRANSIENT RECEPTOR POTENTIAL DROSOPHILA HOMOLOG OF 3 antibody
    • Transient receptor protein 3 antibody
    • Trp related protein 3 antibody
    • TRP3 antibody
    • TrpC3 antibody
    • Trrp3 antibody
    see all

Anti-TRPC3 antibody 画像

  • Immunohistochemistry of TRPC3 in mouse heart tissue with TRPC3 antibody ab51560 at 10 µg/ml.

Anti-TRPC3 antibody (ab51560) 使用論文

This product has been referenced in:
  • Letavernier E  et al. Williams-Beuren syndrome hypercalcemia: is TRPC3 a novel mediator in calcium homeostasis? Pediatrics 129:e1626-30 (2012). ICC/IF, IHC ; Human . Read more (PubMed: 22566418) »
  • Woodard GE  et al. TRPC3 regulates agonist-stimulated Ca2+ mobilization by mediating the interaction between type I inositol 1,4,5-trisphosphate receptor, RACK1, and Orai1. J Biol Chem 285:8045-53 (2010). WB ; Human . Read more (PubMed: 20022948) »

See all 4 Publications for this product

Product Wall

The exact sequence is proprietary information, but we would be happy to run an alignment and forward the results.


Please let us know if you have any more questions.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: dako low pH
Permeabilization No
Blocking step No blocking step used for 30 minute(s) · Concentration: 2%
Username

Dr. mark southwood

Verified customer

投稿 Oct 27 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (platelets)
Loading amount 20 µg
Specification platelets
Treatment Thrombin 0.1U/ml for 3 min
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Dr. Dr Juan A. Rosado

Verified customer

投稿 Apr 07 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"