Anti-TRPC1 抗体 [EP1417Y] (ab51255)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1417Y] to TRPC1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TRPC1 antibody [EP1417Y]
TRPC1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1417Y] to TRPC1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF or IHC -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Human TRPC1.
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ポジティブ・コントロール
- HepG2 whole cell lysate (ab7900).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1417Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51255の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa).
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特記事項 |
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WB
1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 91 kDa). |
ターゲット情報
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機能
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion. -
組織特異性
Seems to be ubiquitous. -
配列類似性
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
Contains 4 ANK repeats. -
翻訳後修飾
Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 7220 Human
- Entrez Gene: 89821 Rat
- Omim: 602343 Human
- SwissProt: P48995 Human
- SwissProt: Q9QX01 Rat
- Unigene: 250687 Human
- Unigene: 684389 Human
- Unigene: 88592 Rat
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別名
- HTRP 1 antibody
- HTRP1 antibody
- MGC133334 antibody
see all
画像
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Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/10000 dilution + HepG2 cell lysate at 10 µg
Secondary
Goat anti-Rabbit HRP labeled at 1/2000 dilution
Predicted band size: 91 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted? -
Anti-TRPC1 antibody [EP1417Y] (ab51255) at 1/500 dilution (for 16 hours) + Rat artery tissue lysate at 50 µg
Secondary
An HRP-conjugated Goat anti-rabbit polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: ~100 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Blocking Step: 5% Milk for 16 hours
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab51255 は 9 報の論文で使用されています。
- Chen X et al. Effect of selective inhibition or activation of PGE2 EP1 receptor on glomerulosclerosis. Mol Med Rep 22:2887-2895 (2020). PubMed: 32700746
- Ye J et al. Tau-induced upregulation of C/EBPß-TRPC1-SOCE signaling aggravates tauopathies: A vicious cycle in Alzheimer neurodegeneration. Aging Cell 19:e13209 (2020). PubMed: 32815315
- Mazzotta C et al. Proangiogenic effects of soluble a-Klotho on systemic sclerosis dermal microvascular endothelial cells. Arthritis Res Ther 19:27 (2017). PubMed: 28183357
- Xu F et al. Effects of TRPC1 on epithelial mesenchymal transition in human airway in chronic obstructive pulmonary disease. Medicine (Baltimore) 96:e8166 (2017). PubMed: 29068985
- Tajeddine N & Gailly P TRPC1 protein channel is major regulator of epidermal growth factor receptor signaling. J Biol Chem 287:16146-57 (2012). WB ; Human . PubMed: 22451676
- Liu XR et al. Enhanced store-operated Ca²+ entry and TRPC channel expression in pulmonary arteries of monocrotaline-induced pulmonary hypertensive rats. Am J Physiol Cell Physiol 302:C77-87 (2012). PubMed: 21940663
- Alessandri-Haber N et al. TRPC1 and TRPC6 channels cooperate with TRPV4 to mediate mechanical hyperalgesia and nociceptor sensitization. J Neurosci 29:6217-28 (2009). WB ; Rat . PubMed: 19439599
- Ulloa A et al. Reduction in TRPC4 expression specifically attenuates G-protein coupled receptor-stimulated increases in intracellular calcium in human myometrial cells. Cell Calcium 46:73-84 (2009). PubMed: 19523685
- Baryshnikov SG et al. Orai1, a critical component of store-operated Ca2+ entry, is functionally associated with Na+/Ca2+ exchanger and plasma membrane Ca2+ pump in proliferating human arterial myocytes. Am J Physiol Cell Physiol 297:C1103-12 (2009). PubMed: 19675303