製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TRPC1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Cow, a wide range of other species
  • 免疫原

    Synthetic peptide derived from the C-terminal domain of TRPC1

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/200 - 1/2000. Predicted molecular weight: 91 kDa.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Seems to be also activated by intracellular calcium store depletion.
  • 組織特異性
    Seems to be ubiquitous.
  • 配列類似性
    Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC1 sub-subfamily.
    Contains 4 ANK repeats.
  • 翻訳後修飾
    Activation of PRKCA induces phosphorylation of TRPC1 and subsequent Ca2+ entry into cells.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HTRP 1 antibody
    • HTRP1 antibody
    • MGC133334 antibody
    • MGC133335 antibody
    • Mtrp1 antibody
    • Short transient receptor potential channel 1 antibody
    • Transient receptor potential canonical 1 antibody
    • Transient receptor potential cation channel subfamily C member 1 antibody
    • Transient receptor potential channel 1 antibody
    • Transient receptor protein 1 antibody
    • TRP 1 antibody
    • TRP 1 protein antibody
    • Trp related protein 1 antibody
    • TRP-1 antibody
    • TRP1 antibody
    • TRP1 protein antibody
    • TRPC1 antibody
    • Trpc1 transient receptor potential cation channel subfamily C member 1 antibody
    • TRPC1_HUMAN antibody
    • Trrp1 antibody
    see all

画像

  • Western blot showing the expression levels of TRPC1, which was significantly reduced in lysates from cells transfected with shTRPC1.
    Briefly, proteins were separated by 10% SDS-PAGE and electrophoretically transferred for 2 hours at 0.8 mA/cm2, in a semi-dry blotter, onto nitrocellulose membranes for subsequent probing. Blots were incubated overnight with 10% (w/v) bovine serum albumin in Tris-buffered saline with 0.1% Tween 20 (TBST) to block residual protein binding sites. ab75322 was diluted 1/200 and incubated in TBST for 2 hours. To detect the primary antibody, blots were incubated for 1 hour with the appropriate horseradish peroxidase-conjugated anti-IgG antibody diluted 1/10,000 in TBST and then exposed to enhanced chemiluminescence reagents for 4 minutes. Blots were then exposed to photographic films. The density of bands on the film was measured using a scanning densitometry.

参考文献

This product has been referenced in:
  • Xia Y  et al. Classical transient receptor potential 1 and 6 contribute to hypoxic pulmonary hypertension through differential regulation of pulmonary vascular functions. Hypertension 63:173-80 (2014). Read more (PubMed: 24144647) »
  • Pullen NA  et al. Novel Mechanism for Fc{epsilon}RI-mediated Signal Transducer and Activator of Transcription 5 (STAT5) Tyrosine Phosphorylation and the Selective Influence of STAT5B over Mast Cell Cytokine Production. J Biol Chem 287:2045-54 (2012). WB ; Mouse . Read more (PubMed: 22130676) »

See all 4 Publications for this product

レビューと Q&A

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Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (hepatocelluar cell carcinoma)
Permeabilization
No
Specification
hepatocelluar cell carcinoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
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投稿 Aug 19 2016

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (liver, Hep G2)
Specification
liver, Hep G2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Jan 19 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8% gel)
Sample
Mouse Tissue lysate - whole (lung)
Specification
lung
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jul 16 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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