製品の概要

  • 製品名Anti-TRPA1 antibody
    TRPA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRPA1
  • アプリケーション適用あり: IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to TRPA1 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab204483)

  • ポジティブ・コントロール
    • DRG cells from human spinal cord

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 5 - 10 µg/ml.

ターゲット情報

  • 機能Receptor-activated non-selective cation channel involved in detection of pain and possibly also in cold perception and inner ear function. Has a central role in the pain response to endogenous inflammatory mediators and to a diverse array of volatile irritants, such as mustard oil, garlic and acrolein, an irritant from tears gas and vehicule exhaust fumes. Acts also as a ionotropic cannabinoid receptor by being activated by delta(9)-tetrahydrocannabinol (THC), the psychoactive component of marijuana. Not involved in menthol sensation. May be a component for the mechanosensitive transduction channel of hair cells in inner ear, thereby participating in the perception of sounds. Probably operated by a phosphatidylinositol second messenger system.
  • 組織特異性Expressed at very low level. Expressed at very low level in human fibroblasts and at a moderate level in liposarcoma cells.
  • 配列類似性Belongs to the transient receptor (TC 1.A.4) family.
    Contains 15 ANK repeats.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ANKTM 1 antibody
    • ANKTM1 antibody
    • Ankyrin-like with transmembrane domains protein 1 antibody
    • Transformation sensitive protein p120 antibody
    • Transformation-sensitive protein p120 antibody
    • Transient receptor potential cation channel subfamily A member 1 antibody
    • TRPA 1 antibody
    • Trpa1 antibody
    • TRPA1_HUMAN antibody
    see all

Anti-TRPA1 antibody 画像

  • ab62053, at 20µg/ml, staining TRPA1 in Formalin/PFA Fixed Paraffin-Embedded Human DRG tissue cells by Immunohistochemistry.
  • ab62053 staining TRPA1 in murine skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in methanol, permeabilized with 0.4% Triton X-100, blocked using 10% serum for 30 minutes at 25°C and then incubated with ab62053 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

Anti-TRPA1 antibody (ab62053) 使用論文

This product has been referenced in:
  • Denda M  et al. Topical application of TRPA1 agonists and brief cold exposure accelerate skin permeability barrier recovery. J Invest Dermatol 130:1942-5 (2010). IHC-Fr ; Mouse . Read more (PubMed: 20182443) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Dako FLEX Peroxidase blocking as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako High pH
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 20 2014

Thank you for your email.

Yes, we do haveone other antibody against TRPA1 which has been tested in frozen sections and on human and mouse tissue - it is the ab62053. http://www.abcam.com/index.html?datasheet=62053 (or use the following: http:...

Read More

Thank you for contacting us. I am very sorry to hear that also with the ab62053 the results are not satisfying. I have looked at the protocol and the only thing I can suggest, is to try also the Methanol fixation. Indeed, the result on the datasheet ha...

Read More

Thank you very much for your reply. Indeed it is as you say. The ab62053 istested in IHC-Fr and covered thereby by our guarantee. In WB, ab62053 is not tested and not guaranteed. However if you provide the feedback, you will get another antibody. Pleas...

Read More

Thank you for providing that information.

As ab58844 failed to work on mouse skin sections in IHC-Fr as guaranteed under our Abpromise, I have sent you ab62053 as a replacement, you order number is 1020816. The replacement antibody is also co...

Read More

Thank you for contacting Abcam.

I am sorry that you are experiencing problems with ab58844.

Would you please be able to provide me with some additional information in case I can make any protocol suggestions. Could you let me know w...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Skin, HR-1)
Specification Skin, HR-1
Fixative Methanol
Permeabilization Yes - 0.4% TritonX100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"