Anti-TRP2 抗体 - N-terminal (ab93473)

製品の概要

  • 製品名Anti-TRP2 antibody - N-terminal
    TRP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRP2 - N-terminal
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide selected from the N terminal region of Human TRP2 conjugated to KLH (NP_001913.2).

  • ポジティブ・コントロール
    • A375 cell line lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab93473 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
  • 追加情報ELISA: 1/1000.
    WB: 1/100 - 1/500. Predicted molecular weight: 59 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Involved in regulating eumelanin and phaeomelanin levels.
    • パスウェイPigment biosynthesis; melanin biosynthesis.
    • 配列類似性Belongs to the tyrosinase family.
    • 細胞内局在Melanosome membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • L dopachrome tautomerase antibody
      • DCT antibody
      • Dopachrome delta isomerase antibody
      • Dopachrome tautomerase antibody
      • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
      • DT antibody
      • EC 5.3.3.12 antibody
      • L dopachrome Delta isomerase antibody
      • L-dopachrome Delta-isomerase antibody
      • L-dopachrome tautomerase antibody
      • TRP 2 antibody
      • TRP-2 antibody
      • TRP2 antibody
      • Tyrosinase related protein 2 antibody
      • Tyrosinase-related protein 2 antibody
      • TYRP2 antibody
      • TYRP2_HUMAN antibody
      see all

    Anti-TRP2 antibody - N-terminal 画像

    • Anti-TRP2 antibody - N-terminal (ab93473) at 1/100 dilution + A375 cell line lysates at 35 µg

      Predicted band size : 59 kDa
    • Anti-TRP2 antibody - N-terminal (ab93473) at 1/2000 dilution + A375 whole cell lysate at 10000 µg

      Secondary
      HRP-conjugated goat anti-rabbit polyclonal IgG at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 59 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

    Anti-TRP2 antibody - N-terminal (ab93473) 使用論文

    ab93473 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (A375 (melanoma))
    Loading amount 10000 cells
    Specification A375 (melanoma)
    Gel Running Conditions Reduced Denaturing (10% acrylamide)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    投稿 May 31 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"