製品の概要

  • 製品名Anti-TRP2 antibody
    TRP2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRP2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 500 to the C-terminus of Human TRP2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: K562; HeLa; A431 and A549.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab92995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 mg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 59 kDa).

ターゲット情報

  • 機能Involved in regulating eumelanin and phaeomelanin levels.
  • パスウェイPigment biosynthesis; melanin biosynthesis.
  • 配列類似性Belongs to the tyrosinase family.
  • 細胞内局在Melanosome membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • L dopachrome tautomerase antibody
    • DCT antibody
    • Dopachrome delta isomerase antibody
    • Dopachrome tautomerase antibody
    • Dopachrome tautomerase dopachrome delta isomerase, tyrosine related protein 2 antibody
    • DT antibody
    • EC 5.3.3.12 antibody
    • L dopachrome Delta isomerase antibody
    • L-dopachrome Delta-isomerase antibody
    • L-dopachrome tautomerase antibody
    • TRP 2 antibody
    • TRP-2 antibody
    • TRP2 antibody
    • Tyrosinase related protein 2 antibody
    • Tyrosinase-related protein 2 antibody
    • TYRP2 antibody
    • TYRP2_HUMAN antibody
    see all

Anti-TRP2 antibody 画像

  • All lanes : Anti-TRP2 antibody (ab92995) at 1 µg/ml

    Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes
  • All lanes : Anti-TRP2 antibody (ab92995) at 1/1000 dilution

    Lane 1 : Human A375 whole cell lysate with 21% Oxygen
    Lane 2 : Human A375 whole cell lysate with 1% Oxygen

    Lysates/proteins at 10000 cells per lane.

    Secondary
    HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 59 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    This image is courtesy of an Abreview submitted by Dr Daniel Tennant

    See Abreview

Anti-TRP2 antibody (ab92995) 使用論文

ab92995 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375 - melanoma cell line)
Loading amount 10000 cells
Specification A375 - melanoma cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Daniel Tennant

Verified customer

投稿 May 31 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"