製品の概要

  • 製品名Anti-Tropomyosin antibody
    Tropomyosin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Tropomyosin
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性

    交差が予測される動物種: Human
  • 免疫原

    Recombinant full length human Tropomyosin protein (AAH11776) with a tag

  • ポジティブ・コントロール
    • Recombinant full length Tropomyosin protein (immunogen).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2a
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab76640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution. Detection limit for ab76640 is approximately 3 ng/ml as a capture antibody.

    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 33 kDa.
    This antibody has only been tested in WB against the recombinant protein used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

  • ターゲット情報

    • 関連性Tropomyosin is a dimeric coiled coil protein that binds along the length of actin filaments. It is associated with the thin filaments of muscle cells and the microfilaments of nonmuscle cells. Chicken embryo fibroblasts (CEF) contain five isoforms of tropomyosin (a, b, 1, 2, and 3), identified as such by their different apparent molecular masses after separation by SDS-PAGE, but similar biochemical properties, such as resistance to heat and organic solvents, the ability to bind to F actin filaments, and the lack of proline and tryptophan.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • Alpha tropomyosin antibody
      • Beta tropomyosin antibody
      • TM30nm antibody
      • TPM1 antibody
      • TPM2 antibody
      • TPM3 antibody
      • Tropomyosin 1 alpha antibody
      • Tropomyosin 1 antibody
      • Tropomyosin 2 antibody
      • Tropomyosin 2 beta antibody
      • Tropomyosin 3 antibody
      see all

    Anti-Tropomyosin antibody 画像

    • Anti-Tropomyosin antibody (ab76640) at 5 µg/ml + immunogen at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 33 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)

    Anti-Tropomyosin antibody (ab76640) 使用論文

    ab76640 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab76640.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"