製品の概要

  • 製品名Anti-Tropomyosin 3 antibody [3D5AH3AB4]
    Tropomyosin 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3D5AH3AB4] to Tropomyosin 3
  • アプリケーション適用あり: IHC-P, IP, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Tissue/ cell preparation

  • ポジティブ・コントロール
    • Human fibroblasts Mouse fibroblasts Rat fibroblasts Human Heart FFPE tissue.
  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
ICC/IF Use a concentration of 2 µg/ml.
Flow Cyt Use 0.1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能Binds to actin filaments in muscle and non-muscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
  • 関連疾患Defects in TPM3 are the cause of nemaline myopathy type 1 (NEM1) [MIM:609284]. A form of nemaline myopathy with autosomal dominant or recessive inheritance. Nemaline myopathies are muscular disorders characterized by muscle weakness of varying severity and onset, and abnormal thread-or rod-like structures in muscle fibers on histologic examination. Autosomal dominant nemaline myopathy type 1 is characterized by a moderate phenotype with onset between birth and early second decade of life. Weakness is diffuse and symmetric with slow progression often with need for a wheelchair in adulthood. The autosomal recessive form has onset at birth with moderate-to-severe hypotonia and diffuse weakness. In the most severe cases, death can occur before 2 years. Less severe cases have delayed major motor milestones, and these patients may walk, but often need a wheelchair before 10 years.
    Defects in TPM3 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TPM3 is found in thyroid papillary carcinomas. A rearrangement with NTRK1 generates the TRK fusion transcript by fusing the amino end of isoform 2 of TPM3 to the 3'-end of NTRK1.
  • 配列類似性Belongs to the tropomyosin family.
  • ドメインThe molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
  • 細胞内局在Cytoplasm > cytoskeleton.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alpha tropomyosin 3 antibody
    • Alpha tropomyosin slow skeletal antibody
    • CFTD antibody
    • Cytoskeletal tropomyosin TM30 antibody
    • FLJ41118 antibody
    • gamma TM antibody
    • Gamma tropomyosin antibody
    • Gamma-tropomyosin antibody
    • Heat stable cytoskeletal protein 30 kDa antibody
    • hscp30 antibody
    • hTM30nm antibody
    • hTM5 antibody
    • hTMnm antibody
    • MGC102590 antibody
    • MGC14582 antibody
    • MGC3261 antibody
    • MGC72094 antibody
    • NEM1 antibody
    • OK/SW-cl.5 antibody
    • OTTHUMP00000034019 antibody
    • OTTHUMP00000034171 antibody
    • OTTHUMP00000034172 antibody
    • TM 5 antibody
    • TM-5 antibody
    • TM3 antibody
    • TM30 antibody
    • TM30nm antibody
    • TM5 antibody
    • Tm5NM antibody
    • Tpm 5 antibody
    • TPM3 antibody
    • TPM3/NTRK1 FUSION GENE, INCLUDED antibody
    • TPM3_HUMAN antibody
    • Tpm5 antibody
    • TPMsk3 antibody
    • TRK antibody
    • TRK ONCOGENE, INCLUDED antibody
    • Trop 5 antibody
    • Tropomyosin 3 antibody
    • Tropomyosin 3 gamma antibody
    • Tropomyosin 5 antibody
    • Tropomyosin alpha 3 chain antibody
    • Tropomyosin alpha-3 chain antibody
    • Tropomyosin gamma antibody
    • Tropomyosin-3 antibody
    • Tropomyosin-5 antibody
    see all

Anti-Tropomyosin 3 antibody [3D5AH3AB4] 画像

  • Overlay histogram showing A431 cells stained with ab113692 (red line). The cells were fixed with 80% methanol (5 min)/ and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113692, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692) at 1 µg/ml

    Lane 1 : HepG2 (human)
    Lane 2 : HDFN (human)
    Lane 3 : H9C2 (rat)
    Lane 4 : H4IIE (rat)
    Lane 5 : MEF (mouse)

    Lysates/proteins at 20 µg per lane.

    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
  • ab113692 stained human fibroblast (HDFn) cells, rat cardiomyocytes (H9C2 cells) and mouse embryo fibroblast (MEF) cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody (3D5AH3AB4,5 µg/ml) for 2h at room temperature or over night at 4°C. The secondary antibody was (red) Alexa Fluor® 594 goat anti-mouse IgG2b (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. The target protein locates to cytoskeleton.
  • IHC image of Tropomyosin 3 staining in Human heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113692, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-Tropomyosin 3 antibody [3D5AH3AB4] (ab113692) 使用論文

This product has been referenced in:
  • Singleton DC  et al. Hypoxic regulation of RIOK3 is a major mechanism for cancer cell invasion and metastasis. Oncogene 0:N/A (2014). Read more (PubMed: 25486436) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse, brain tissue, whole brain sections)
Specification Mouse, brain tissue, whole brain sections
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - Tween-20
Blocking step Heat-inactivated normal donkey serum in 0.05% PBS-T as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Mar 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"