製品の概要

  • 製品名Anti-TRMT5 antibody
    TRMT5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRMT5
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 460-509 (EMLCITFQIP ASVLYKNQTR NPENHEDPPL KRQRTAEAFS DEKTQIVSNT) of human TRMT5 (NP_065861).

  • ポジティブ・コントロール
    • MCF7 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83379 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Specifically methylates guanosine-37 in various tRNAs. Not dependent on the nature of the nucleoside 5' of the target nucleoside.
  • 配列類似性Belongs to the TRM5 / TYW2 family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA1393 antibody
    • M1G methyltransferase antibody
    • M1G-methyltransferase antibody
    • TRM5 antibody
    • TRM5 tRNA methyltransferase 5 homolog (S. cerevisiae) antibody
    • TRMT5 antibody
    • TRMT5_HUMAN antibody
    • tRNA (guanine-N(1)-)-methyltransferase antibody
    • tRNA [GM37] methyltransferase antibody
    • tRNA methyltransferase 5 antibody
    • tRNA N1G37 methyltransferase antibody
    see all

Anti-TRMT5 antibody 画像

  • Anti-TRMT5 antibody (ab83379) at 1 µg/ml (in 5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 58 kDa
    Observed band size : 67 kDa (why is the actual band size different from the predicted?)

Anti-TRMT5 antibody (ab83379) 使用論文

ab83379 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab83379.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"