製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TrkC
  • 特異性
    From experiments using similar antisera, it is anticipated that the reagent will block TrkC receptor binding of corresponding Nerve Growth factor ligand.
  • アプリケーション
    適用あり: ICC/IF, IHC-Frmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Extracellular domain of Human TrkC.

  • ポジティブ・コントロール
    • Rat peripheral sensory ganglia.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab43078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/250.
IHC-Fr 1/3000.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Receptor for neurotrophin-3 (NT-3). This is a tyrosine-protein kinase receptor. Known substrates for the trk receptors are SHC1, PI-3 kinase, and PLCG1. The different isoforms do not have identical signaling properties.
    • 組織特異性
      Widely expressed but mainly in nervous tissue. Isoform B is expressed at higher levels in adult brain than in fetal brain.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Ligand-mediated auto-phosphorylation.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC 2.7.10.1 antibody
      • ETS related protein neurotrophic receptor tyrosine kinase fusion antibody
      • ETS related protein neurotrophic receptor tyrosine kinase fusion protein antibody
      • ETV6 NTRK3 fusion antibody
      • GP145 TrkC antibody
      • gp145(trkC) antibody
      • GP145-TrkC antibody
      • GP145TrkC antibody
      • Neurotrophic tyrosine kinase receptor type 3 antibody
      • Neurotrophin 3 receptor antibody
      • NT 3 growth factor receptor antibody
      • NT 3 growth factor receptor precursor antibody
      • NT 3 receptor antibody
      • NT-3 growth factor receptor antibody
      • Ntrk3 antibody
      • NTRK3_HUMAN antibody
      • OTTHUMP00000192915 antibody
      • TRK C antibody
      • Trk-C antibody
      • TRKC antibody
      • TrkC tyrosine kinase antibody
      • Tyrosine kinase receptor C antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence analysis of human U2OS cells labeling TrkC with ab43078 at 1/250. Cells were fixed in formaldehyde and permeabilized with 3% milk powder + 0.1% Triton X-100 in PBS. Blocking of the cells was done with 3% milk for 1 hour at 22°C, followed by staining with ab43078 at 1/250 in 3% milk powder+0.1% Triton X-100 in PBS for 1 hour at 22°C. A FITC conjugated goat anti-rabbit secondary antibody was used at 1/400 dilution.

      Figure shows expression and cellular locaization of endogenous TrkC in U2OS cells. DAPI nuclear stain is also shown in adjacent picture.

      See Abreview

    参考文献

    This product has been referenced in:
    • Zong H  et al. A Hyperactive Signalosome in Acute Myeloid Leukemia Drives Addiction to a Tumor-Specific Hsp90 Species. Cell Rep 13:2159-73 (2015). Read more (PubMed: 26628369) »
    • Cesca F  et al. Kidins220/ARMS mediates the integration of the neurotrophin and VEGF pathways in the vascular and nervous systems. Cell Death Differ 19:194-208 (2012). Read more (PubMed: 22048155) »

    See all 2 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Endogenous TrkC expression in U2OS)
    Specification
    Endogenous TrkC expression in U2OS
    Fixative
    Formaldehyde
    Permeabilization
    Yes - 3% milk powder+0.1% Triton X-100 in PBS
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

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    投稿 Nov 19 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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