製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to TrkB
  • 特異性
    From experiments using similar antisera, it is anticipated that the reagent will block TrkB receptor binding of corresponding Nerve Growth factor ligand.
  • アプリケーション
    適用あり: ELISA, IHC-Frmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Extracellular domain of Mouse TrkB.

  • ポジティブ・コントロール
    • Rat peripheral sensory ganglia.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab43079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
IHC-Fr 1/3000.
  • 追加情報
    Is unsuitable for WB.
  • ターゲット情報

    • 機能
      Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC-gamma-1.
    • 組織特異性
      Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
    • 配列類似性
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Ligand-mediated auto-phosphorylation.
    • 細胞内局在
      Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI848316 antibody
      • BDNF tropomyosine receptor kinase B antibody
      • BDNF/NT 3 growth factors receptor antibody
      • BDNF/NT-3 growth factors receptor antibody
      • Brain derived neurotrophic factor receptor antibody
      • C030027L06Rik antibody
      • EC 2.7.10.1 antibody
      • GP145 TrkB antibody
      • GP145-TrkB antibody
      • GP145-TrkB/GP95-TrkB antibody
      • GP95 TrkB antibody
      • Neurotrophic receptor tyrosine kinase 2 antibody
      • Neurotrophic tyrosine kinase receptor type 2 antibody
      • Neurotrophin receptor tyrosine kinase type 2 antibody
      • NTRK 2 antibody
      • Ntrk2 antibody
      • NTRK2_HUMAN antibody
      • Obesity, hyperphagia, and developmental delay, included antibody
      • RATTRKB1 antibody
      • Tkrb antibody
      • Trk B antibody
      • Trk-B antibody
      • TRKB antibody
      • TrkB tyrosine kinase antibody
      • TRKB1 antibody
      • Tropomyosin related kinase B antibody
      • tyrosine kinase receptor B antibody
      • Tyrosine receptor kinase B antibody
      see all

    画像

    • The cryostat section of the rat spinal cord was incubated in rabbit polyclonal antibodies to the extracellular domain of mouse TrkB at the dilution of 1/3000 overnight followed with a biotinylated secondary antibodies and ABC kit. The section was developed in diaminobenzidine solution and enhanced in the presence of nickel sulphate. Nerve fibres in the ventral horn are stained.

    プロトコール

    参考文献

    ab43079 has not yet been referenced specifically in any publications.

    レビューと Q&A

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Rat Tissue sections (adult brain: cerebrum, hippocampus)
    Specification
    adult brain: cerebrum, hippocampus
    Fixative
    acetone-methanol (2:3)
    Permeabilization
    No
    Blocking step
    ProteinBlock (DakoCytomation) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Angelika Richter

    Verified customer

    投稿 Oct 25 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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