Anti-TrkA + TrkB 抗体 [EPR1104(2)] (ab109010)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-TrkA + TrkB antibody [EPR1104(2)] -
製品の詳細
Rabbit monoclonal [EPR1104(2)] to TrkA + TrkB -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human fetal brain and SH SY5Y lysates; Human brain tissue.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1104(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109010の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 87 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 87 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. |
ターゲット情報
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細胞内局在
TrkA: Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity). TrkB: Membrane. -
参照データベース
- Entrez Gene: 4914 Human
- Entrez Gene: 4915 Human
- Entrez Gene: 18211 Mouse
- Entrez Gene: 18212 Mouse
- Entrez Gene: 25054 Rat
- Entrez Gene: 59109 Rat
- Omim: 191315 Human
- Omim: 600456 Human
see all
画像
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All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1.118 µg/ml (purified)
Lanes 1 & 3 & 5 : Empty vector overexpression 293T cell lysates
Lane 2 : His-Human TrkC overexpression 293T cell lysates
Lane 4 : His-Human TrkA overexpression 293T cell lysates
Lane 6 : His-Human TrkB overexpression 293T cell lysates
Lane 7 : Human brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 87 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?Blocking/Diluting Buffer and concentration: 5% NFDM /TBST
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ab109010 at 1/250 dilution staining TrkA + TrkB in Human brain by Immunohistochemistry, Paraffin-embedded tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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All lanes : Anti-TrkA + TrkB antibody [EPR1104(2)] (ab109010) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 87 kDa -
ab109010 showing negative staining in Normal human kidney tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab109010 showing negative staining in Normal human liver tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab109010 showing negative staining in Normal human tonsil tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab109010 showing negative staining in Normal human colon tissue. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab109010 は 1 報の論文で使用されています。
- Peng T et al. Nerve Growth Factor (NGF) Encourages the Neuroinvasive Potential of Pancreatic Cancer Cells by Activating the Warburg Effect and Promoting Tumor Derived Exosomal miRNA-21 Expression. Oxid Med Cell Longev 2022:8445093 (2022). PubMed: 36285300