製品の概要

  • 製品名Anti-TrkA antibody
    TrkA 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TrkA
  • 特異性When used for immunohistochemistry in Rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is, in small, nociceptive neurons. From experiments using similar antisera, it is anticipated that the reagent will block TrkA receptor binding of corresponding Nerve Growth factor ligand.
  • アプリケーション適用あり: ELISA, IHC-Fr, Dot blotmore details
    適用なし: WB
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Extracellular domain of Mouse TrkA.

  • ポジティブ・コントロール
    • Rat dorsal root ganglia.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab36961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/5000.
IHC-Fr 1/2000.
Dot blot 1/2000.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand, it can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors.
      Isoform TrkA-III is resistant to NGF, constitutively activates AKT1 and NF-kappa-B and is unable to activate the Ras-MAPK signaling cascade. Antagonizes the anti-proliferative NGF-NTRK1 signaling that promotes neuronal precursors differentiation. Isoform TrkA-III promotes angiogenesis and has oncogenic activity when overexpressed.
    • 組織特異性Isoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
    • 関連疾患Congenital insensitivity to pain with anhidrosis
      Chromosomal aberrations involving NTRK1 are found in papillary thyroid carcinomas (PTCs) (PubMed:2869410, PubMed:7565764, PubMed:1532241). Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1 (PubMed:7565764). A rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1 (PubMed:2869410). An intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein (PubMed:1532241).
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 2 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 protein kinase domain.
    • ドメインThe transmembrane domain mediates interaction with KIDINS220.
      The extracellular domain mediates interaction with NGFR.
    • 翻訳後修飾Ligand-mediated autophosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent. Autophosphorylation at Tyr-496 mediates interaction and phosphorylation of SHC1.
      N-glycosylated (Probable). Isoform TrkA-I is N-glycosylated.
      Ubiquitinated. Undergoes polyubiquitination upon activation; regulated by NGFR. Ubiquitination regulates the internalization of the receptor.
    • 細胞内局在Cell membrane. Early endosome membrane. Late endosome membrane. Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes (By similarity).
    • Information by UniProt
    • 参照データベース
    • 別名
      • gp140trk antibody
      • High affinity nerve growth factor receptor antibody
      • High affinity nerve growth factor receptor precursor antibody
      • MTC antibody
      • Neurotrophic tyrosine kinase receptor type 1 antibody
      • NTRK1 antibody
      • NTRK1_HUMAN antibody
      • Oncogene TRK antibody
      • p14-TrkA antibody
      • p140 TrkA antibody
      • p140-TrkA antibody
      • Slow nerve growth antibody
      • Trk A antibody
      • TRK antibody
      • Trk-A antibody
      • TRK1 antibody
      • TRK1-transforming tyrosine kinase protein antibody
      • Tropomyosin-related kinase A antibody
      • Tyrosine kinase receptor A antibody
      • Tyrosine kinase receptor antibody
      see all

    Anti-TrkA antibody 画像

    • ab36961 staining Rat TrkA in dorsal root ganglia by immunohistochemistry (Frozen sections) at 1/2000 dilution. Staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons.The cryostat section of the rat spinal cord was incubated in rabbit polyclonal antibodies to the extracellular domain of mouse Trk A overnight followed with a biotinylated secondary antibodies and ABC kit. The section was developed in diaminobenzidine solution and enhanced in the presence of nickel sulphate. Nerve fibres in the dorsal horn are stained.

    Anti-TrkA antibody (ab36961) 使用論文

    ab36961 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Spinal cord)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Specification Spinal cord
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    投稿 Dec 21 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"