製品の概要

  • 製品名Anti-TRK fused gene antibody
    TRK fused gene 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRK fused gene
  • アプリケーション適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to region between residues 175 and 225 of human TRK fused gene (NP_001007566.1)

  • ポジティブ・コントロール
    • HeLa, 293T and NIH3T3 whole cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 組織特異性Ubiquitous.
  • 関連疾患Defects in TFG are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving TFG is found in thyroid papillary carcinomas. Translocation t(1;3)(q21;q11) with NTRK1. The TFG sequence is fused to the 3'-end of NTRK1 generating the TRKT3 (TRK-T3) fusion transcript.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ36137 antibody
    • HMSNP antibody
    • OTTHUMP00000214045 antibody
    • OTTHUMP00000214046 antibody
    • OTTHUMP00000214047 antibody
    • OTTHUMP00000214048 antibody
    • Protein TFG antibody
    • SPG57 antibody
    • TF6 antibody
    • TFG antibody
    • TFG_HUMAN antibody
    • TRK fused antibody
    • TRK fused gene antibody
    • TRK fused gene protein antibody
    • TRK-fused gene protein antibody
    • TRKT3 antibody
    • TRKT3 oncogene antibody
    see all

Anti-TRK fused gene antibody 画像

  • All lanes : Anti-TRK fused gene antibody (ab86606) at 1/0.1 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 44 kDa


    Exposure time : 30 seconds
  • Detection of TRK fused gene by Western Blot of Immunprecipitate.
    ab86606 at 1µg/ml staining TRK fused gene in HeLa whole cell lysate immunoprecipitated using ab86606 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.
  • ICC/IF image of ab86606 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86606, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-TRK fused gene antibody (ab86606) 使用論文

ab86606 has not yet been referenced specifically in any publications.

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