製品の概要

  • 製品名Anti-Tristetraprolin antibody
    Tristetraprolin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Tristetraprolin
  • 特異性Does not react with Mouse, based on IHC-P Abreview.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Sheep, Rabbit, Guinea pig, Cow, Cat, Dog非交差種: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Tristetraprolin aa 84-133.
    Sequence:

    PLAPRLGPELSPSPTSPTATSTTPSRYKTELCRTFSESGRCRYGAKCQFA


    Database link: P26651
    (Peptide available as ab111664)

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab33058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).Can be blocked with Human Tristetraprolin peptide (ab111664). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 µg/ml.

ターゲット情報

  • 機能mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF).
  • 配列類似性Contains 2 C3H1-type zinc fingers.
  • 翻訳後修飾Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation.
  • 細胞内局在Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules.
  • Information by UniProt
  • 参照データベース
  • 別名
    • G0/G1 switch regulatory protein 24 antibody
    • G0S24 antibody
    • GOS24 antibody
    • Growth factor-inducible nuclear protein NUP475 antibody
    • NUP475 antibody
    • Protein TIS11A antibody
    • RNF162A antibody
    • TIS 11 antibody
    • TIS11 antibody
    • TIS11A antibody
    • Tristetraprolin antibody
    • Tristetraproline antibody
    • TTP antibody
    • TTP_HUMAN antibody
    • Zfp-36 antibody
    • ZFP36 antibody
    • Zinc finger protein 36 antibody
    • Zinc finger protein 36 homolog antibody
    • Zinc finger protein 36, C3H type, homolog (mouse) antibody
    • Zinc finger protein, C3H type, 36 homolog antibody
    see all

Anti-Tristetraprolin antibody 画像

  • Lane 1 : Anti-Tristetraprolin antibody (ab33058) at 2 µg/ml
    Lane 2 : Anti-Tristetraprolin antibody (ab33058) at 1 µg/ml
    Lane 3 : Anti-Tristetraprolin antibody (ab33058) at 0.5 µg/ml

    Lane 1 : Cell lysate prepared from human HepG2 cells
    Lane 2 : Cell lysate prepared from human HepG2 cells
    Lane 3 : Cell lysate prepared from human HepG2 cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 36 kDa
    Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
  • ab33058 (4µg/ml) staining Tristetraprolin in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic and nuclei of macrophages and nuclei staining in some pneumocytes. There was also cytoplasmic and nuclei staining of epithelium cells of the bronchioles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab33058 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab33058, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Tristetraprolin antibody (ab33058) 使用論文

This product has been referenced in:
  • Chowdhury S  et al. IL-17 Attenuates Degradation of ARE-mRNAs by Changing the Cooperation between AU-Binding Proteins and microRNA16. PLoS Genet 9:e1003747 (2013). Read more (PubMed: 24086143) »
  • Shi JX  et al. MK2 post-transcriptionally regulates TNF-a-induced expression of ICAM-1 and IL-8 via tristetraprolin in human pulmonary microvascular endothelial cells. Am J Physiol Lung Cell Mol Physiol : (2012). WB ; Human . Read more (PubMed: 22268119) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (Mast cells)
Loading amount 300000 cells
Specification Mast cells
Treatment LPS 100ng/ml, 0, 10 and 60 minutes
Gel Running Conditions Reduced Denaturing (10)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Jan 28 2013

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for taking time to provide this information and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

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Thank you for contacting us.

I am sorry to hear that this product is not performing as expected. This product is covered by our Abpromise guarantee. If this is used according to the specification listed on the datasheet (on human in IHC-P,...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab124024.

To check the status of the order please contact ...

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Thank you for contacting Abcam regarding ab14371 and ab33058. I have checked the datasheets for both of these antibodies, and they have not yet been tested in EMSA. Therefore, we do not know if these products will work in this assay and cannot guar...

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Currently we have two anti-TTP antibodies, however, neither of them are specific to the phosphorylated form of the protein. I am sorry that I could not be of more help. Please feel free to contact us again should you have further questions about the pr...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (intestine)
Specification intestine
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization No
Blocking step BSA and serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
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Verified customer

投稿 Oct 11 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (macrophages)
Loading amount 25000 cells
Specification macrophages
Gel Running Conditions Reduced Denaturing (NuPage 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Mar 04 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"