Anti-Tristetraprolin/TTP 抗体 [OTI8B5] (ab124024)
Key features and details
- Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
- Reacts with: Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-Tristetraprolin/TTP antibody [OTI8B5]
Tristetraprolin/TTP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [OTI8B5] to Tristetraprolin/TTP -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human Tristetraprolin/TTP.
Database link: NP_003398 -
ポジティブ・コントロール
- HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA; Human colon, colon adenocarcinoma, ovary, endometrium and prostate tissues; COS7 cells transiently transfected by pCMV6-ENTRY Tristetraprolin/TTP.
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特記事項
Dilute in PBS (pH7.3) before use.
The clone number has been updated from 8B5 to OTI8B5, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
OTI8B5 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124024の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50.
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ICC/IF |
1/100.
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Flow Cyt (Intra) |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1 - 1/1000.
|
特記事項 |
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IHC-P
1/50. |
ICC/IF
1/100. |
Flow Cyt (Intra)
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
1/1 - 1/1000. |
ターゲット情報
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機能
mRNA-binding protein involved in post-transcriptional regulation of AU-rich element (ARE)-containing mRNAs. Acts by specifically binding ARE-containing mRNAs and promoting their degradation. Recruits deadenylase CNOT7 (and probably the CCR4-NOT complex) via association with CNOT1. Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). Plays a key role in the post-transcriptional regulation of tumor necrosis factor (TNF). -
配列類似性
Contains 2 C3H1-type zinc fingers. -
翻訳後修飾
Phosphorylation by MAPKAPK2 increases its stability and binding to 14-3-3 proteins, leading to reduce its ARE affinity leading to inhibition of degradation of ARE-containing transcripts. Phosphorylated upon mitogen stimulation. -
細胞内局在
Nucleus. Cytoplasm. Localizes to stress granules upon energy starvation. phosphorylation by MAPKAPK2 promotes exclusion from stress granules. - Information by UniProt
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参照データベース
- Entrez Gene: 7538 Human
- Omim: 190700 Human
- SwissProt: P26651 Human
- Unigene: 534052 Human
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別名
- G0/G1 switch regulatory protein 24 antibody
- G0S24 antibody
- GOS24 antibody
see all
画像
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All lanes : Anti-Tristetraprolin/TTP antibody [OTI8B5] (ab124024)
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected with pCMV6-ENTRY Tristetraprolin/TTP cDNA
Lysates/proteins at 5 µg per lane.
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC202049) -
ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human ovary tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human endometrium tissue by Immunohistochemistry.
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ab124024, at 1/50, staining Tristetraprolin/TTP in paraffin embedded Human prostate tissue by Immunohistochemistry.
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ab124024, at 1/100, staining Tristetraprolin/TTP in COS7 cells transiently transfected with pCMV6-ENTRY Tristetraprolin/TTP by Immunofluorescence.
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ab124024 at 1/100 dilution staining Tristetraprolin/TTP in HEK293T cells transfected with either pCMV6-ENTRY Tristetraprolin/TTP (Red) or empty vector control plasmid (Blue) by Flow Cytometry (Intracellular).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab124024 は 4 報の論文で使用されています。
- Fakhfakh R et al. Differential Expression of Anti-Inflammatory RNA Binding Proteins in Lupus Nephritis. Life (Basel) 12:N/A (2022). PubMed: 36294909
- Wang M et al. Nicotine-mediated OTUD3 downregulation inhibits VEGF-C mRNA decay to promote lymphatic metastasis of human esophageal cancer. Nat Commun 12:7006 (2021). PubMed: 34853315
- Na L et al. Ultrashort Wave Combined with Human Umbilical Cord Mesenchymal Stem Cell (HUC-MSC) Transplantation Inhibits NLRP3 Inflammasome and Improves Spinal Cord Injury via MK2/TTP Signalling Pathway. Biomed Res Int 2020:3021750 (2020). PubMed: 33376718
- Selmi T et al. ZFP36 stabilizes RIP1 via degradation of XIAP and cIAP2 thereby promoting ripoptosome assembly. BMC Cancer 15:357 (2015). PubMed: 25939870