Anti-TRIP13 抗体 - Carboxyterminal end (ab64964)


  • 製品名Anti-TRIP13 antibody - Carboxyterminal end
    TRIP13 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to TRIP13 - Carboxyterminal end
  • 特異性Detects endogenous levels of total TRIP13 protein.
  • アプリケーション適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide derived from C terminal of human TRIP13.

  • ポジティブ・コントロール
    • Extracts from LOVO cells and NIH 3T3 cells IHC-P: Human lunf FFPE tissue sections.


  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野


Our Abpromise guarantee covers the use of ab64964 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA 1/5000.
WB 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).


  • 配列類似性Belongs to the AAA ATPase family.
  • Information by UniProt
  • 参照データベース
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Anti-TRIP13 antibody - Carboxyterminal end 画像

  • IHC image of TRIP13 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-TRIP13 antibody - Carboxyterminal end (ab64964) at 1/500 dilution

    Lane 1 : extracts from LOVO cells without immunizing peptide
    Lane 2 : extract from NIH 3T3 cells, without immunizing peptide
    Lane 3 : extract from NIH 3T3 cells with immunizing peptide

    Predicted band size : 49 kDa
    Observed band size : 49 kDa
    The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

Anti-TRIP13 antibody - Carboxyterminal end (ab64964) 使用論文

ab64964 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (12% SDS-PAGE)
Sample Human Cell lysate - whole cell (NIH 3T3)
Specification NIH 3T3
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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投稿 Aug 28 2014