Anti-TRIP13 抗体 - Carboxyterminal end (ab64964)
製品の概要
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製品名Anti-TRIP13 antibody - Carboxyterminal end
TRIP13 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to TRIP13 - Carboxyterminal end
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由来種Rabbit
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特異性Detects endogenous levels of total TRIP13 protein.
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アプリケーション適用あり: IHC-P, ELISA, WBmore details
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種交差性交差種: Mouse, Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide derived from C terminal of human TRIP13.
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ポジティブ･コントロール
- Extracts from LOVO cells and NIH 3T3 cells IHC-P: Human lunf FFPE tissue sections.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab64964 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ELISA | 1/5000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
ターゲット情報
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配列類似性Belongs to the AAA ATPase family.
- Information by UniProt
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参照データベース
- Entrez Gene: 9319 Human
- Entrez Gene: 69716 Mouse
- Entrez Gene: 292206 Rat
- Omim: 604507 Human
- SwissProt: Q15645 Human
- SwissProt: Q3UA06 Mouse
- SwissProt: Q5XHZ9 Rat
- Unigene: 275095 Mouse
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別名
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画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIP13 antibody - Carboxyterminal end (ab64964)IHC image of TRIP13 staining in human lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64964, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-TRIP13 antibody - Carboxyterminal end (ab64964)All lanes : Anti-TRIP13 antibody - Carboxyterminal end (ab64964) at 1/500 dilution
Lane 1 : extracts from LOVO cells without immunizing peptide
Lane 2 : extract from NIH 3T3 cells, without immunizing peptide
Lane 3 : extract from NIH 3T3 cells with immunizing peptide
Predicted band size: 49 kDa
Observed band size: 49 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
参考文献
This product has been referenced in:
- Pressly JD et al. TRIP13-deficient tubular epithelial cells are susceptible to apoptosis following acute kidney injury. Sci Rep 7:43196 (2017). Read more (PubMed: 28256593) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"